The aim of this research work was to investigate the interaction of loteprednol etabonate (LE) loaded poly (D, L- lactide co-glycolide) (PLGA) polymer based nanoparticles with ocular mucosa ex vivo. Rhodamine (Rd) was used as a fluorescent marker compound to prepare Rd-LE-PLGA-NPs. The formulation was characterized for various parameters like particle size, polydispersity index (PDI), zeta potential, XRD, DSC, surface morphology, drug entrapment and ex-vivo permeation profile. The prepared formulation was evaluated for their penetration profile in freshly excised goat cornea using Franz diffusion apparatus and confocal laser scanning microscopy (CLSM). The integrity of the comeal tissue was investigated by optical digital microscopy of the histopathological slide stained with hematoxylene and eosin dye. The result clearly showed that drug loaded nanoparticle exhibited better permeation profile as compared to plain drug suspension. Additionally, less intensity of fluorescence was observed from drug suspension as compared to LE-PLGA-NPs. There was uniform and intense florescence observed across the total depth of excised goat corneal tissue, suggesting improved penetration profile of nanoparticles, which can be attributed to the nano size range of the formulation and precorneal retent of the nanocarrier. Ex vivo permeation showed prolonged release pattern from NPs for up to 4 hours. The entrapment efficiency and mean diameter was found to be 96.3121.68 % and 167.620.37 nm respectively. The TEM confirmed the nano dimension of the particles. The results advocate that the prepared novel nanoparticulates could be a potential carrier for ophthalmic therapeutics.
Cite this article:
Sah and Suresh (2016). Penetration Study of Loteprednol Etabonate Loaded Polymeric Nanoparticle across the Excise Goat Cornea: A Study with Confocal Microscopy. Journal of Ravishankar University (Part-B: Science), 29(1), pp.74-75.