Abstract View

Author(s): Beeta Rani Khalkho, Anushree Saha, Bhuneshwari Sahu, Manas Kanti Deb*

Email(s): debmanas@yahoo.com

Address: School of Studies in Chemistry, Pt. Ravishankar Shukla University, Raipur-492010, Chhattisgarh, India

Published In:   Volume - 34,      Issue - 1,     Year - 2021

DOI: 10.52228/JRUB.2021-34-1-6  

ABSTRACT:
Abstract. The purpose of the present research was to design a method for the colorimetric determination of L-cysteine. We have employed PVA capped gold nanoparticles (GNPs) as a probe. The as-synthesized GNPs were further characterized by UV-vis absorption spectroscopy, transmission electron microscope (TEM), Fourier transform infrared spectroscopy (FTIR), dynamic light scattering (DLS) and Zeta potential analyser. The results show that the presence of L-cysteine caused the quenching of the surface plasmon resonance band of the GNPs at 524 nm. It was accompanied by the appearance of a new absorbance of a new absorbance band at 670 nm. The color of the colloidal GNPs changed from wine red to blue. The change in color of the GNPs was due to their aggregation induced by the presence of L-cysteine. Based on these observations, the as-synthesized GNPs were utilized to develop a novel colorimetric sensor for L-cysteine detection in food samples. Significantly, other biomolecules such as alanine, proline, phenylalanine, tryptophane, valine, arginine, glutamic acid, lysine and histidine did not cause any change in the color of the GNPs solutions. This colorimetric probe showed excellent selectivity and high sensitivity for L-cysteine with a detection limit of 2.0 μg mL-1.

Cite this article:
Khalkho et al. (2021). Simple and Cost Effective Polymer Modified Gold Nanoparticles Based on Colorimetric Determination of L-Cysteine in Food Samples. Journal of Ravishankar University (Part-B: Science), 34(1), pp. 41-57.DOI: https://doi.org/10.52228/JRUB.2021-34-1-6


Abbas MN, Saeed AA, Singh B, Radowan AA, Dempsey E (2015). A cysteine sensor based on a gold nanoparticle-iron phthalocyanine modified graphite paste electrode, Anal. Methods, 7, 2529-2536

Agui L, Farfal CP, Sedeno PY, Pinngarron JM (2007). Electrochemical determination of homocysteine at a gold nanoparticle-modified electrode, Talanta,74, 412-420.

Amarnath K, Amarnath V, Amarnath K, Valentine HL, Valentine WM (2003).  A specific HPLC-UV method for the determination of cysteine and related aminothiols in biological samples, Talanta, 60, 1229-1238.

Anand T, Sivaraman G, Chellappa D (2014). Hg2+ mediated quinazoline ensemble for highly selective recognition of cysteine, Spectrochim. Acta A Mol. Biomol. Spectrosc. 123, 18-24.

Bamdad F, Khorram F, Samet M, Bamdad K, Sangi MR, Allahbakhshi F (2016). Spectrophotometric determination of L-cysteine by using polyvinylpyrrolidone-stabilized silver nanoparticles in the presence of barium ions, Spectrochim. Acta A Mol. Biomol. Spectrosc. 161, 52–57.

Chai F, Wang C, Wang T (2010). L-cysteine functionalized gold nanoparticles for the colorimetric detection of Hg2+ induced by ultraviolet light, Nanotechnology, 21, 25501.

Chaichi MJ, Ehsani M, Khajvand T, Golchoubian H, Rezaee E (2014).  Determination of cysteine and glutathione based on the inhibition of the dinuclear Cu(II)-catalyzed luminol–H2O2 chemiluminescence reaction, Spectrochim. Acta A Mol. Biomol. Spectrosc. 122, 405-410.

Chen S, Gao H, Shen W, Lu C, Yuan Q (2014). Colorimetric detection of cysteine using noncrosslinking aggregation of fluorosurfactant-capped silver nanoparticles, Sens. Actuators B Chem. 190 673-678.

Chen S, Gao H, Shen W, Lu C, Yuan Q (2014). Colorimetric detection of cysteine using noncrosslinking aggregation of fluorosurfactant-capped silver nanoparticles, Sens. Actuators B, 190, 673-678.

Chen Y, Qin X, Yuan C, Wang Y (2020) Switch on fluorescence mode for determination of L-cysteine with carbon quantum dots and Au nanoparticles as a probe RSC Adv., 10, 1989-1994.

Cui M, Song G, Wang C, Song Q (2015). Synthesis of cysteine-functionalized water-soluble luminescent copper nanoclusters and their application to the determination of chromium(VI), Microchim. Acta, 182, 1371-1377.

Denga HH, Wu CL, Liu AL, Li GW, Chen W, Lin XH (2014). Colorimetric sensor for thiocyanate based on anti-aggregation ofcitrate-capped gold nanoparticles, Sens. Actuators B, 191, 479-484.

Haghnazari N, Alizadeh A, Karami C, Hamidi Z (2013). Simple optical determina-tion of silver ion in aqueous solutions using benzo crown-ether modified gold nanoparticles, Microchim Acta, 180, 287-294.

Hajizadeh S, Farhadi K, Forough M, Molaei R (2012). Silver nanoparticles in the presence of Ca2+ as a selective and sensitive probe for the colorimetric detection of cysteine, Anal. Methods, 4, 1747-1752.

Jiang Y, Zhao H, Liu YQ, Zhu NN, Ma YR, Mao LQ (2010) Angew. Chem. Int. Ed. 49, 4800-4804.

Kargosha K, Ahmadi SH, Zeeb M, Moeinossadat SR (2008). Vapour phase Fourier transform infrared spectrometric determination of l-cysteine and l-cystine, Talanta 74, 753-759.

Kataoka H, Takagi K, Makita M (1995), Determination of total plasma homocysteine and related aminothiols by gas chromatography with flame photometric detection, J. Chromatogr. B Biomed. Appl. 664, 421-425.

Khan Z, Singh T, Hussain JI, Hashmi AA (2013). Au(III)-CTAB reduction by ascorbic acid: Preparation and characterization of gold Nanoparticles, Colloids Surf. B, 104, 11-17.

Li Y, Schluesener HJ, Xu S (2010). Gold nanoparticle-based biosensors, Gold Bull. 43 29-41.

Mahendia S, Tomar AK, Goyal PK, Kumar S (2013). Tuning of refractive index of poly(vinyl alcohol): Effect of embedding Cu and Ag nanoparticles, J. Appl. Phys. 113, 073103.

Mocanua A, Cernica I, Tomoaia G, Bobos LD, Horovitz O, Cotisel MT (2009). Self-assembly characteristics of gold nanoparticles in the presence of cysteine, Colloids and Surfaces A: Physicochem. Eng. Aspects, 338, 93-101.

Nidya M, Umadevi M, Rajkumar BJM (2014). Structural, morphological and optical studies of L-cysteine modified silver nanoparticles and its application as a probe for the selective colorimetric detection of Hg2+, Spectrochim. Acta A Mol. Biomol. Spectrosc. 133, 265-271.

Panigrahi S, Basu S, Praharaj S, Pande S, Jana S, Pal A, Ghosh SK, Pal T (2007). Synthesis and Size-Selective Catalysis by Supported Gold Nanoparticles: Study on Heterogeneous and Homogeneous Catalytic Process, J. Phys. Chem. C, 111, 4596-4605.

Ravindran A, Mani V, Chandrasekaran N, Mukherjee A (2011). Selective colorimetric sensing of cysteine in aqueous solutions using silver nanoparticles in the presence of Cr3+ , Talanta, 85, 533-540.

Saha K, Agasti SS, Kim C, Li XN, Rotello VM (2012). Gold nanoparticles in chemical and biological sensing, Chem. Rev. 112, 2739-2779.

Santhoshkumar CR, Deutsch JC, Kolhouse JC, Hassell KL, Kolhouse JF (1994). Measurement of excitatory sulfur amino acids, cysteine sulfinic acid, cysteic acid, homocysteine sulfinic acid, and homocysteic acid in serum by stable isotope dilution gas chromatography-mass spectrometry and selected ion monitoring, Anal. Biochem. 220, 249-256.

Shrivas  KSahu J, Majia P, Sinha D (2017). Label-free selective detection of ampicillin drug in human urine samples using silver nanoparticles as a colorimetric sensing probe, New J. Chem. 41, 6685-6692.

Smitha SL, Nissamudeen KM, Philip D, Gopchandran KG (2008). Studies on surface plasmon resonance and photoluminescence of silver nanoparticles, Spectrochimica Acta Part A, 71, 186-190.

Soomro RA, Nafady A, Sirajuddin, Memon N, Sherazi TH, Kalwara NH (2014). L-cysteine protected copper nanoparticles as colorimetric sensor for mercuric ions, Talanta, 130, 415-422.

Xinfu M, Qingquan G, Yu X, Haixiang M (2016). Green chemistry for the preparation of L-cysteine functionalized silver nanoflowers, Chem. Phys. Lett. 652, 148-151.

Zhang J, Xu XW, Yang XR (2012). Highly specific colorimetric recognition and sensing of sulfide with glutathione-modified gold nanoparticle probe based on an anion-for-molecule ligand exchange reaction, Analyst, 137, 1556-1558.

Related Images:



Recent Images



Modeling of Abnormal Hysteresis in CsPbBr3 based Perovskite Solar Cells
Need of Gallium Recovery from Waste Samples: A Review
Higher Order Statistics Based Blind Steg analysis using Deep Learning
Covid-19 related School Closure Impact on School going Children & Adolescents of Raipur, Chhattisgarh
Determination of Pentachlorophenol in Environmental Samples by Spectrophotometry
Simple and Cost Effective Polymer Modified Gold Nanoparticles Based on Colorimetric Determination of L-Cysteine in Food Samples
Performance Evaluation of Spectrogram Based Epilepsy Detection Techniques Using Gray Scale Features
Perovskite Solar Cells an Efficient, Low Cost, Emerging Photovoltaic Technology
Spectrophotometric Determination of Phenthoate in Vegetables and Fruit Samples of Kabirdham (Chhattisgarh)
Flotation-Dissolution-Spectrophotometric Determination of Phorate in Various Environmental Samples

Tags


Recomonded Articles:

Author(s): Ajay kumar Sahu; Shraddha Ganesh Pandey; Vindhya Patel; Raisa Khatoon; Mamta Nirmal; Kalpana Wani; Deepak Kumar Sahu; Jyoti Goswami; Chhaya Bhatt; Geetanjali Deshlahare; Harshita Sharma; Manish Kumar Raia* and Joyce Rai

DOI: 10.52228/JRUB.2018-31-1-5         Access: Open Access Read More

Author(s): Deepti Tikariha; Jyotsna Lakra; Srishti Dutta Roy; Toshikee Yadav; Kallol KGhosh

DOI:         Access: Open Access Read More

Author(s): Manmohan L Satnami; Kuleshwar Patel; Sandeep K Vaishnav; Kumudini Chandraker; Jyoti Korram; Rekha Nagwanshi; Kallol K Ghosh

DOI:         Access: Open Access Read More

Author(s): Beeta Rani Khalkho; Anushree Saha; Bhuneshwari Sahu; Manas Kanti Deb*

DOI: 10.52228/JRUB.2021-34-1-6         Access: Open Access Read More