Author(s):
Jagriti Chandrakar, Neeraj Agrawal, Lowkesh Chandravanshi, Satyawati Rathia, Arun KS Parihar, Chandan Kumar Sahu, Nagendra Singh Chauhan
Email(s):
Chauhan.nagendra@gmail.com
Address:
Department of Kaumarabhritya, Shri Narayan Prasad Awasthi Government Ayurved College Raipur (C.G.), India.
Drugs Testing Laboratory AvamAnusandhan Kendra, Raipur, Chhattisgarh 402010, India.
*Corresponding Author: Chauhan.nagendra@gmail.com
Published In:
Volume - 38,
Issue - 1,
Year - 2025
DOI:
10.52228/JRUB.2025-38-1-10 
ABSTRACT:
The present study focuses on the HPTLC estimation of piperine in Drakshadi Ghrita. The method involves the extraction of piperine from the Drakshadi Ghrita formulation, followed by its separation and quantification using an HPTLC system. The analysis is performed on a silica gel 60 F254 plate, using a suitable mobile phase for the optimal separation of piperine. The HPTLC analysis was performed using a developing solvent system consisting of toluene, ethyl acetate, and formic acid in a ratio of 7:3:0.3 (v/v/v). The HPTLC method accurately quantified piperine in Drakshadi Ghrita with Rf values of 0.43. The technique showed results presented as the coefficient of variance of 24.03. When the plate is visualized under UV light, specific wavelengths detect piperine due to its unique fluorescence properties. The HPTLC method developed offers a reliable approach for the quality control and standardization of Drakshadi Ghrita, facilitating consistent potency and ensuring its therapeutic efficacy.
Cite this article:
Chandrakar, Agrawal, Chandravanshi, Rathia, Parihar, Sahu, and Chauhan (2025). Quantitative Estimation of Piperine in Drakshadi Ghrita by HPTLC Method. Journal of Ravishankar University (Part-B: Science), 38(1), pp. 143-150. DOI:DOI: https://doi.org/10.52228/JRUB.2025-38-1-10
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