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Author(s): Tarun Kumar Patle, Kamlesh Shrivas, Reena Jamunkar, Antresh Kumar, Khushali Tandey

Email(s): kshrivas@gmail.com

Address: School of Studies in Chemistry, Pt. Ravishankar Shukla University, Raipur-492010, CG, India.
Department of Chemistry, Government Nagarjuna Post Graduate College of Science, Raipur-492010, CG, India.
Department of Biochemistry, Central University of Haryana, Jant-Pali Mahendergarh-123029, HR, India.
*Corresponding author: kshrivas@gmail.com

Published In:   Volume - 38,      Issue - 1,     Year - 2025

DOI: 10.52228/JRUB.2025-38-1-11  

ABSTRACT:
Green leafy vegetables play a crucial role in the diet of the tribal communities in Central India, offering significant nutritional and medicinal benefits. This study investigates the phytochemical composition of Cordia dichotoma, Hibiscus sabdariffa, Marsilea vestita, and Portulaca oleracea, focusing on phenolic and flavonoid content using ultrasound-assisted extraction (UAE). Various UAE parameters, including vessel position, depth, frequency, time, and solvent ratio, were optimized to enhance extraction efficiency. Antioxidant activity was assessed using UV-Vis spectroscopy, while gallic acid and ellagic acid were quantified via reverse phase-high performance liquid chromatography-diode array detector (RP-HPLC-DAD). Fourier-transform infrared (FTIR) spectroscopy identified functional groups and analyzed polyphenol composition in the extracts. The results showed that UAE significantly improved the yield of total phenolic content (TPC) and total flavonoid content (TFC). H. sabdariffa exhibited the highest TPC (16.897±0.052 mg GAE/g) and TFC (92.522±0.081 mg RUE/g), while C. dichotoma had the lowest levels. DPPH radical scavenging activity ranged from 80.648±1.332% to 89.416±1.753% at 10 µg/mL extract, confirming strong antioxidant potential. This study highlights UAE as a sustainable method for extracting bioactive compounds, reinforcing the nutritional and pharmacological value of these vegetables as natural antioxidants for health benefits.

Cite this article:
Patle, Shrivas, Jamunkar, Kumar, and Tandey (2025). Screening of phenolics and flavonoids using FTIR and UV-Vis: Antioxidant activity and HPLC quantification of gallic acid and ellagic acid. Journal of Ravishankar University (Part-B: Science), 38(1), pp. 151-171. DOI:DOI: https://doi.org/10.52228/JRUB.2025-38-1-11


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