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Author(s): Sonam Patel, Afreen Anjum, Veenu Joshi, Afaque Quraishi

Email(s): drafaque13@gmail.com

Address: School of Studies in Biotechnology, Pt. Ravishankar Shukla University, Raipur, C.G., India.
School of Studies in Biotechnology, Pt. Ravishankar Shukla University, Raipur, C.G., India.
Center for Basic Sciences, Pt. Ravishankar Shukla University, Raipur, C.G., India.
School of Studies in Biotechnology, Pt. Ravishankar Shukla University, Raipur, C.G., India.
*Corresponding author: drafaque13@gmail.com

Published In:   Volume - 37,      Issue - 1,     Year - 2024

DOI: 10.52228/JRUB.2024-37-1-4  

ABSTRACT:
Curcuma caesia Roxb. is a highly valuable, endangered herb of therapeutic importance that resides in their rhizomes. In the present investigation, the effect of ½ strength liquid Murashige and Skoog (MS) medium supplemented with 1 mg/l Indole-3-butyric acid (IBA) and different sucrose concentrations (1.5%, 3%, 6%, 9%, or 12%) was studied on microrhizomes induction of C. caesia. The shoot length, root length and microrhizomes dry weight of C. caesia decreased significantly at 6% sucrose and above. When compared to the control (1.5% sucrose), the current water content significantly decreased at 6% sucrose. The optimum concentration for in vitro microrhizomes induction in C. caesia was 6% sucrose. Therefore for further experiments, the 6% sucrose was used. We also studied the effect of silver nanoparticles (AgNP) on microrhizome induction and antioxidant activity in C. caesia cultures. Field-grown C. caesia rhizomes extract was used in the green synthesis of AgNP. The synthesized AgNP was further characterized through scanning electron microscopy and X-ray diffraction. The AgNP, ranging from 0, 0.025, 0.05, 0.075 or 0.1 mg/l was supplemented in ½ strength liquid MS medium with 6% sucrose & 1 mg/l IBA. The MS medium with 0.05 mg/l AgNP found with significant morphological changes in C. caesia cultures (root number, root length and microrhizomes fresh weight). For the total phenolic and total terpenoids content estimation as well as for antioxidant activity analysis, the extracts of un-treated cultures (6% sucrose + 1 mg/l IBA, without AgNP), AgNP treated cultures (6% sucrose + 1 mg/l IBA with 0.025 & 0.05 mg/l AgNP) was used. The 0.025 and 0.05 mg/l AgNP enhanced the phenolic and terpenoid content in the cultures compared to the field-grown mother plant. The antioxidant activity of the cultures treated with AgNP also increased compared to un-treated cultures and field-grown mother plant. The Gas Chromatography-Mass Spectrometry (GC-MS) analysis revealed that the extract treated with 0.05 mg/l AgNP had increased production of monoterpene (camphor) and sesquiterpenes (β-elemenone & curcumenone). These increased terpenes could be responsible for the enhanced antioxidant activity of C. caesia cultures.

Cite this article:
Patel, Anjum, Joshi and Quraishi (2024). Enhanced antioxidant activity in Curcuma caesia Roxb. microrhizomes treated with silver nanoparticles. Journal of Ravishankar University (Part-B: Science), 37(1), pp. 49-71. DOI:DOI: https://doi.org/10.52228/JRUB.2024-37-1-4


References

Ahmad, M.B., Shameli, K., Darroudi, M., Wan Yunus, W.M.Z., and Ibrahim, N.A. (2009). Synthesis and characterization of silver/clay nanocomposites by chemical reduction method. American Journal of Applied Sciences, 6: 1909-1914.

Ainsworth, E.A., and Gillespie, K.M. (2007). Estimation of total phenolic content and other oxidation substrates in plant tissues using Folin–Ciocalteu reagent. Nature Protocols, 2: 875-877.

Anjum, A., and Quraishi, A. (2023). Enhanced epicurzerenone production via in vitro elicitation of microrhizomes of Curcuma caesia Roxb. In Vitro Cellular & Developmental Biology-Plant, 59: 1-14.

Anjum, A., Singh, V., Adil, S., and Quraishi, A. (2022). In vitro propagation of Curcuma caesia Roxb. via bud culture technique and ISSR profiling of the plantlets for genetic homogeneity. Research Journal of Biotechnology, 17: 48-54.

Baghel, S., Baghel, R., Sharma, K., and Sikarwar, I. (2013). Pharmacological activities of Curcuma caesia. International Journal of Green Pharmacy, 7: 1-5.

Benya, A., Mohanty, S., Hota, S., Das, A.P., Rath, C.C., Achary, K.G., and Singh, S. (2023). Endangered Curcuma caesia Roxb.: Qualitative and quantitative analysis for identification of industrially important elite genotypes. Industrial Crops and Products195: 116363.

Blois, M.S. (1958). Antioxidant determinations by the use of a stable free radical. Nature. 181: 1199- 1200.

Borah, A., Paw, M., Gogoi, R., Loying, R., Sarma, N., Munda, S., Pandey, S.K., and Lal, M. (2019). Chemical composition, antioxidant, anti-inflammatory, anti-microbial and in-vitro cytotoxic efficacy of essential oil of Curcuma caesia Roxb. leaves: An endangered medicinal plant of North East India. Industrial crops and products129: 448-454.

Chauhan, R., Keshavkant, S., and Quraishi, A. (2018). Enhanced production of diosgenin through elicitation in micro-tubers of Chlorophytum borivilianum Sant. et Fernand. Industrial Crops and Products, 113: 234-239.

Chirangini, P., Sinha, S.K., and Sharma, G.J. (2005). In vitro propagation and microrhizome induction in Kaempferia galangal Linn. and K. rotunda Linn. Indian Journal of Biotechnology, 4:.404-408.

Chung, I.M., Rekha, K., Rajakumar, G., and Thiruvengadam, M. (2018). Elicitation of silver nanoparticles enhanced the secondary metabolites and pharmacological activities in cell suspension cultures of bitter gourd. 3 Biotech, 8: 1-2.

Cittrarasu, V., Balasubramanian, B., Kaliannan, D., Park, S., Maluventhan, V., Kaul, T., Liu, W.C., and Arumugam, M. (2019). Biological mediated Ag nanoparticles from Barleria longiflora for antimicrobial activity and photocatalytic degradation using methylene blue. Artificial Cells, Nanomedicine, and Biotechnology47: 2424-2430.

Donipati, P., and Sreeramulu, S.H. (2015). In vitro bioevaluation of antioxidant activity in Curcuma longa.      International Journal of Innovative Pharmaceutical Sciences and Research 3: 1238-1243.

Elegbede, J.A., Lateef, A., Azeez, M.A., Asafa, T.B., Yekeen, T.A., Oladipo, I.C Adebayo, E.A., Beukes, L.S.,  and Gueguim‐Kana, E.B. (2018). Fungal xylanases‐mediated synthesis of silver nanoparticles for catalytic and biomedical applications. IET Nanobiotechnology, 12: 857-863.

Geoprincy, G., Srri, B.V., Poonguzhali, U., Gandhi, N.N., and Renganathan, S. (2013). A review on green synthesis of silver nanoparticles. Asian Journal of Pharmaceutical and Clinical Research, 6: 8-12.

Ghorai, N., Chakraborty, S., Gucchait, S., Saha, S.K., and Biswas, S. (2012). Estimation of total terpenoids concentration in plant tissues using a monoterpene, Linalool as standard reagent. Protocol Exchange, 5: 1-5.

Hasan, M., Sajjad, M., Zafar, A., Hussain, R., Anjum, S.I., Zia, M., Ihsan, Z., and Shu, X. (2022). Blueprinting morpho-anatomical episodes via green silver nanoparticles foliation. Green Processing and Synthesis, 11: 697-708.

Karmakar, I., Dolai, N., Saha, P., Sarkar, N., Bala, A., and Haldar, P.K. (2011.) Scavenging activity of Curcuma caesia rhizome against reactive oxygen and nitrogen species. Oriental Pharmacy and Experimental Medicine, 11: 221-228.

Keshari, A.K., Srivastava, R., Singh, P., Yadav, V.B., and Nath, G. (2020). Antioxidant and antibacterial activity of silver nanoparticles synthesized by Cestrum nocturnum. Journal of Ayurveda and Integrative Medicine 11: 37-44.

Khattab, S., Alkuwayti, M.A., Yap, Y.K., Meligy, A.M., Bani Ismail, M., and El Sherif, F. (2023). Foliar spraying of ZnO nanoparticals on Curcuma longa had increased growth, yield, expression of curcuminoid synthesis genes, and curcuminoid accumulation. Horticulturae, 9: 355.

Kim, S.H., and Kim, S.K. (2002). Effect of sucrose level and nitrogen source on fresh weight and anthocyanin production in cell suspension culture of ‘Sheridan’ Grape (Vitis spp). Journal of Plant Biotechnology, 4: 2327-2330.

Logeswari, P., Silambarasan, S., and Abraham, J. (2013). Ecofriendly synthesis of silver nanoparticles  from commercially available plant powders and their antibacterial properties. Scientia Iranica, 20: 1049-1054.

Mamidi, G., and Polaki, S.J. (2019). Synthesis and characterization of biogenic silver nanoparticles and its antimicrobial analysis. Journal of Applied Chemistry, 8: 112-123.

Mehta, U.J., Krishnamurthy, K.V., and Hazra, S. (2000). Regeneration of plants via adventitious bud formation from mature zygotic embryo axis of tamarind (Tamarindus indica L.). Current Science, 78: 1231-1234.

Mukunthan, K.S., Balaji, B., and Patel, T.N. (2018). Black turmeric database: A database of natural compounds from Curcuma caesia Roxb. Asian Journal of Pharmaceutical and Clinical Research, 11: 406-408.

Murashige, T., and Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue culture. Plant Physiology, 15: 473-497.

Naik, R.R., Stringer, S.J., Agarwal, G., Jones, S.E., and Stone, M.O. (2002). Biomimetic synthesis and patterning of silver nanoparticles. Nature Materials, 1: 169-172.

Nayak, S., and Naik, P.K. (2006). Factors effecting in vitro microrhizome formation and growth in Curcuma longa L. and improved field performance of micropropagated plants. Science Asia, 32: 31-37.

Oyaizu, M. (1986). Studies on products of browning reaction antioxidative activities of products of browning reaction prepared from glucosamine. The Japanese Journal of Nutrition and Dietetics, 44: 307-315.

Ravindran, P.N., Babu, K.N., and Sivaraman, K. (2007). Turmeric: The genus Curcuma. London, Chemical Rubber Company press.

Salih, A.M., Qahtan, A.A., Al-Qurainy, F., and Al-Munqedhi, B.M. (2022). Impact of biogenic ag-containing nanoparticles on germination rate, growth, physiological, biochemical parameters, and antioxidants system of tomato (Solanum tuberosum L.) in vitroProcesses10: 825.

Sami, F., Yusuf, M., Faizan, M., Faraz, A., and Hayat, S. (2016). Role of sugars under abiotic stress. Plant Physiology and Biochemistry, 109: 54-61.

Selvan, D.A., Mahendiran, D., Kumar, R.S., and Rahiman, A.K. (2018). Garlic, green tea and turmeric extracts-mediated green synthesis of silver nanoparticles: Phytochemical, antioxidant and in vitro cytotoxicity studies. Journal of Photochemistry and Photobiology B: Biology180: 243-252.

Shameli, K., Ahmad, M.B., Jazayeri, S.D., Shabanzadeh, P., Sangpour, P., Jahangirian, H., and Gharayebi, Y. (2012). Investigation of antibacterial properties silver nanoparticles prepared via green method. Chemistry Central Journal, 6: 73.

Solanki, S., Lakshmi, G.B., Dhiman, T., Gupta, S., Solanki, P.R., Kapoor, R., and Varma, A. (2023). Co-application of silver nanoparticles and symbiotic fungus Piriformospora indica improves secondary metabolite production in black rice. Journal of Fungi, 9: 260.

Stepanov, A.L. (1997). Optical properties of metal nanoparticles synthesized in a polymer by ion implantation: A review. Technical Physics, 49: 143-153.

Valentovic, P., Luxova, M., Kolarovic, L., and Gasparikova, O. (2006). Effect of osmotic stress on compatible solutes content, membrane stability and water relations in two maize cultivars. Plant Soil and Environment, 52: 184.

Zhang, W., and Jiang, W. (2020). Antioxidant and antibacterial chitosan film with tea polyphenols-mediated green synthesis silver nanoparticle via a novel one-pot method. International Journal of Biological Macromolecules155: 1252-1261.

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