Abstract View

Author(s): Shatabdi Ghose, Umamaheswari S, Susithra E, Rajasekhar Chekkara, Naresh Kandakatla, Uma Maheswara Reddy C

Email(s): shagh08@gmail.com , esusithra@gmail.com , rajasekhar@gmail.com , sparkshabz@gmail.com

Address: Department of Pharmacology, Faculty of Pharmacy, Sri Ramachandra University. Chennai
Department of Pharmacognosy, Faculty of Pharmacy, Sri Ramachandra University, Chennai
Department of Chemistry, Satyabama University, Jeppiar Nagar, Chennai, India.

Published In:   Volume - 29,      Issue - 1,     Year - 2016

DOI: Not Available

ABSTRACT:
Secondary metabolites from natural sources play a crucial role in the treatment of various ailments in humans. Traditionally, Operculina turpethum has been used to treat gastrointestinal disturbances and asthma. Therapeutically, the plant has activity against tumor, tuberculosis, malaria, etc. In the present study, the scavenging capacity of the chloroform extract was studied as a prelude to the anti-neoplastic efficacy. The molecular simulation studies to identify the bioactive constituents in Operculina turpethum responsible for anticancer property were done. Further, the chloroform extract was assessed for its anti-oxidant potential. The eight naturally occurring molecules were identified by GC-MS analysis namely, 3-(4-hydroxy-phenyl)-N-[2-(4-hydroxyphenyl)-ethyl)-acrylamide: Stigma-5,22dien-3-0-b-D-glucopyranoside; Turpethinic acids A-E, and were docked against various cancer proteins namely, JNKI (PDB ID:4L7F), MMP-9 (PDB ID:4XCT). Caspase-3 (PDB ID 2XYG), PARP-1 (PDB ID:4UND), ERK2 (PDB ID:3C9W), AKTI (PDB ID:4EKL) and CDK6 (PDB ID:4TTH) using LigandFit module in Discovery Studio. The chloroform extract showed significant anti-oxidant property with IC value of 126.58ug/ml. From molecular docking studies, it was observed that all the docked compounds showed effective hydrogen bond interactions with the active amino acid residues present in the active domain of proteins like, INKI, MMP-9, Caspase-3, PARP-1, ERK2& CDK6. Furthermore the "Stigma-5, 22dien-3-0-b-D-glucopyranoside" docked compound shows good H-bond interactions and bond length with the active residues present in the active domain of PARP-1. ERK2 and AKTI. From the above study, it is inferred that the chloroform extract possessed significant anti-oxidant property and among the molecules docked, 3-(4-hydroxy-phenyl)-N-[2-(4-hydroxy phenyl)-ethyl]-acrylamide, Turpethinic acids C and Turpethinic acids E showed more interaction score and H-bond formation with all the docked cancer proteins. It can be concluded that a rational drug designing with the above targeted compounds might be useful in development of selective inhibitors for treatment of cancer.

Cite this article:
Ghose, S, E, Chekkara, Kandakatla and C (2016). Pharmacological Screening of the Bioactive Constituents of Operculina turpethum. Journal of Ravishankar University (Part-B: Science), 29(1), pp.101-102.


References not available.

Related Images:



Recent Images



Optimized Structural Design of G+7 Residential Buildings: A Modern Approach with ETABS
Quantitative Estimation of Piperine in Drakshadi Ghrita by HPTLC Method
Screening of phenolics and flavonoids using FTIR and UV-Vis: Antioxidant activity and HPLC quantification of gallic acid and ellagic acid
Carbon Dots in Biomedical Applications: A Review of Their Interaction with Serum Albumins, Antidepressant Agents, and Enzymatic Systems
Review on Solar Energy in India, Achievements (2021–2025), and Benefits of Solar Energy
Investigation on Groundwater Quality in Sukma Bastar District, Chhattisgarh
Implement of Dijkstra method in Village data management system for Optimal Route Calculation
Fiber Reinforced Concrete: Comparative Analysis of Helix Steel Fibers and Plain Steel Fibers in Structural Applications
Dynamic Geometrical Modeling and Computational Analysis of Multi-Bob Pendulum Wave Interference Systems
A Comparative Analysis of Inventory Models: Evaluating the Economic Order Quantity (EOQ) Model with Constant Demand versus Variable Demand Rates

Tags


Recomonded Articles:

Author(s): Suman Shrivastava, Pooja Deshpande, S. J. Daharwal*

DOI: 10.52228/JRUB.2018-31-1-6         Access: Open Access Read More

Author(s): Deepak Kumar Sahu; Joyce Rai; Chhaya Bhatt; Manish K. Rai*; Jyoti Goswami; Ajay Kumar Sahu; Thakur Vikram Singh; Mamta Nirmala; Kalpana Wani and Prashant Mundeja

DOI: 10.52228/JRUB.2018-31-1-1         Access: Open Access Read More

Author(s): Sunandan Mandal; Kavita Thakur; Bikesh Kumar Singh; Heera Ram

DOI: 10.52228/JRUB.2020-33-1-1         Access: Open Access Read More

Author(s): Suchita Agrawal; Prabha Rohatgi

DOI:         Access: Open Access Read More

Author(s): Swati Jain; Somesh Kumar Dewangan

DOI: 10.52228/JRUB.2021-34-1-9         Access: Open Access Read More

Author(s): Pooja Deshpande, Suman Shrivastava, S.J. Daharwal*

DOI: 10.52228/JRUB.2018-31-1-7         Access: Open Access Read More

Author(s): Krishna Yadav*; Jyoti Pawar; Deependra Singh; Manju Rawat Singh

DOI: 10.52228/JRUB.2018-31-1-2         Access: Open Access Read More

Author(s): B GopalKrishna; Sanjay Tiwari

DOI: 10.52228/JRUB.2021-34-1-1         Access: Open Access Read More

Author(s): Monika Swami; Kinjal Patel

DOI: 10.52228/JRUB.2021-34-1-2         Access: Open Access Read More

Author(s): Surendra G Gattani; Ravina Shete; Sandeep Ambore

DOI:         Access: Open Access Read More

Author(s): Preeti Verma*; S. K. Chatterjee; Sanjay Ghosh; Deepak Sinha

DOI: 10.52228/JRUB.2020-33-1-8         Access: Open Access Read More

Author(s): Rajesh Shukla; Neetu Harmukh; Nayan Kumar Pandey

DOI:         Access: Open Access Read More

Author(s): Chhaya Bhatt*; Deepak Kumar Sahua; Thakur Vikram Singh; Kalpana Wani; Jyoti Goswami; Ajay Kumar Sahu; Harshita Sharma; Geetanjali Deshlehre; Manish Kumar Rai*; Joyce Rai.

DOI: 10.52228/JRUB.2020-33-1-2         Access: Open Access Read More

Author(s): Lokesh Kumar Sahu*; Vaishali Soni*

DOI: 10.52228/JRUB.2022-35-1-1         Access: Open Access Read More

Author(s): Anil Kumar Verma*; Swati Sahu; Mohan Patel; Sanjay Tiwari

DOI: 10.52228/JRUB.2020-33-1-5         Access: Open Access Read More