Abstract View

Author(s): Shatabdi Ghose, Umamaheswari S, Susithra E, Rajasekhar Chekkara, Naresh Kandakatla, Uma Maheswara Reddy C

Email(s): shagh08@gmail.com , esusithra@gmail.com , rajasekhar@gmail.com , sparkshabz@gmail.com

Address: Department of Pharmacology, Faculty of Pharmacy, Sri Ramachandra University. Chennai
Department of Pharmacognosy, Faculty of Pharmacy, Sri Ramachandra University, Chennai
Department of Chemistry, Satyabama University, Jeppiar Nagar, Chennai, India.

Published In:   Volume - 29,      Issue - 1,     Year - 2016

DOI: Not Available

ABSTRACT:
Secondary metabolites from natural sources play a crucial role in the treatment of various ailments in humans. Traditionally, Operculina turpethum has been used to treat gastrointestinal disturbances and asthma. Therapeutically, the plant has activity against tumor, tuberculosis, malaria, etc. In the present study, the scavenging capacity of the chloroform extract was studied as a prelude to the anti-neoplastic efficacy. The molecular simulation studies to identify the bioactive constituents in Operculina turpethum responsible for anticancer property were done. Further, the chloroform extract was assessed for its anti-oxidant potential. The eight naturally occurring molecules were identified by GC-MS analysis namely, 3-(4-hydroxy-phenyl)-N-[2-(4-hydroxyphenyl)-ethyl)-acrylamide: Stigma-5,22dien-3-0-b-D-glucopyranoside; Turpethinic acids A-E, and were docked against various cancer proteins namely, JNKI (PDB ID:4L7F), MMP-9 (PDB ID:4XCT). Caspase-3 (PDB ID 2XYG), PARP-1 (PDB ID:4UND), ERK2 (PDB ID:3C9W), AKTI (PDB ID:4EKL) and CDK6 (PDB ID:4TTH) using LigandFit module in Discovery Studio. The chloroform extract showed significant anti-oxidant property with IC value of 126.58ug/ml. From molecular docking studies, it was observed that all the docked compounds showed effective hydrogen bond interactions with the active amino acid residues present in the active domain of proteins like, INKI, MMP-9, Caspase-3, PARP-1, ERK2& CDK6. Furthermore the "Stigma-5, 22dien-3-0-b-D-glucopyranoside" docked compound shows good H-bond interactions and bond length with the active residues present in the active domain of PARP-1. ERK2 and AKTI. From the above study, it is inferred that the chloroform extract possessed significant anti-oxidant property and among the molecules docked, 3-(4-hydroxy-phenyl)-N-[2-(4-hydroxy phenyl)-ethyl]-acrylamide, Turpethinic acids C and Turpethinic acids E showed more interaction score and H-bond formation with all the docked cancer proteins. It can be concluded that a rational drug designing with the above targeted compounds might be useful in development of selective inhibitors for treatment of cancer.

Cite this article:
Ghose, S, E, Chekkara, Kandakatla and C (2016). Pharmacological Screening of the Bioactive Constituents of Operculina turpethum. Journal of Ravishankar University (Part-B: Science), 29(1), pp.101-102.


References not available.

Related Images:



Recent Images



A Review on role of Congestion Control Techniques in Internet of Things
Recent Advancement in Capsule: Emerging Novel Technologies and Alternative Shell Materials for Wide Range of Therapeutic Needs
Effect of L-Dopa on cypermethrin induced reproductive conditions in female Japanese quail, Coturnix coturnix japonica
Preparation, Characterization, and Applications of Albumin Serum-Based Nanoparticles
Study of developmental stages and morphometrics of Parthenium beetle in Bastar plateau agro-climatic zone of Chhattisgarh
PANI Incorporated Fe-MOF: As an Electrode Material for Supercapacitor
Surface Modified Magnetic Nanoparticles as an Efficient Material for Wastewater Remediation: A Review
A Review on Groundwater Pollution in India and their Health Problems
Incidence of Chronic fever in Raigarh Development Block of Raigarh District, Chhattisgarh, India
Study the optimization of Dijkstra’s Algorithm

Tags


Recomonded Articles:

Author(s): Deepak Kumar Sahu; Joyce Rai; Chhaya Bhatt; Manish K. Rai*; Jyoti Goswami; Ajay Kumar Sahu; Thakur Vikram Singh; Mamta Nirmala; Kalpana Wani and Prashant Mundeja

DOI: 10.52228/JRUB.2018-31-1-1         Access: Open Access Read More

Author(s): Suman Shrivastava, Pooja Deshpande, S. J. Daharwal*

DOI: 10.52228/JRUB.2018-31-1-6         Access: Open Access Read More

Author(s): Sunandan Mandal; Kavita Thakur; Bikesh Kumar Singh; Heera Ram

DOI: 10.52228/JRUB.2020-33-1-1         Access: Open Access Read More

Author(s): Swati Jain; Somesh Kumar Dewangan

DOI: 10.52228/JRUB.2021-34-1-9         Access: Open Access Read More

Author(s): Suchita Agrawal; Prabha Rohatgi

DOI:         Access: Open Access Read More

Author(s): Pooja Deshpande, Suman Shrivastava, S.J. Daharwal*

DOI: 10.52228/JRUB.2018-31-1-7         Access: Open Access Read More

Author(s): Krishna Yadav*; Jyoti Pawar; Deependra Singh; Manju Rawat Singh

DOI: 10.52228/JRUB.2018-31-1-2         Access: Open Access Read More

Author(s): B GopalKrishna; Sanjay Tiwari

DOI: 10.52228/JRUB.2021-34-1-1         Access: Open Access Read More

Author(s): Monika Swami; Kinjal Patel

DOI: 10.52228/JRUB.2021-34-1-2         Access: Open Access Read More

Author(s): Surendra G Gattani; Ravina Shete; Sandeep Ambore

DOI:         Access: Open Access Read More

Author(s): Preeti Verma*; S. K. Chatterjee; Sanjay Ghosh; Deepak Sinha

DOI: 10.52228/JRUB.2020-33-1-8         Access: Open Access Read More

Author(s): Chhaya Bhatt*; Deepak Kumar Sahua; Thakur Vikram Singh; Kalpana Wani; Jyoti Goswami; Ajay Kumar Sahu; Harshita Sharma; Geetanjali Deshlehre; Manish Kumar Rai*; Joyce Rai.

DOI: 10.52228/JRUB.2020-33-1-2         Access: Open Access Read More

Author(s): Akhilesh Kumar Tiwari*

DOI: 10.52228/JRUB.2019-32-1-7         Access: Open Access Read More

Author(s): Anil Kumar Verma*; Swati Sahu; Mohan Patel; Sanjay Tiwari

DOI: 10.52228/JRUB.2020-33-1-5         Access: Open Access Read More

Author(s): Anushree Saha; Manas Kanti Deb*; Mithlesh Mahilang; Shubhra Sinha

DOI: 10.52228/JRUB.2020-33-1-7         Access: Open Access Read More