Author(s):
Chhaya Bhatt*, Deepak Kumar Sahua, Thakur Vikram Singh, Kalpana Wani, Jyoti Goswami, Ajay Kumar Sahu, Harshita Sharma, Geetanjali Deshlehre, Manish Kumar Rai*, Joyce Rai.
Email(s):
chhaya05bhatt@gmail.com , mkjkchem@gmail
Address:
School of Studies in Chemistry, Pt. Ravishankar Shukla University, Raipur (Chhattisgarh), 492010, India
School of Studies in Environmental Science, Pt. Ravishankar Shukla University, Raipur (Chhattisgarh), 492010, India
Chhattisgarh Council of Science and Technology,MIG-25, Indrawati Colony,Raipur (Chhattisgarh),492007, India.
Published In:
Volume - 33,
Issue - 1,
Year - 2020
DOI:
10.52228/JRUB.2020-33-1-2 
ABSTRACT:
A large variety of pesticides have been used in the agriculturearea to raise the quality, extend and yield storage life of crops.However, the use of pesticides has been greater than before now a day dueto the growing rapid urbanization and population. The nonstop uses ofthese pesticides have resulted in pollution of the environment and alsocaused risk to human health. For the rapid detection of selective phenthoate pesticides, we developed a simple and highly sensitive spectrophotometric method based on azo-coupling reaction. After a simple pretreatment of phenthoatewith a diazotized solution,the resulting is rapidly undergoing azo-coupling reaction with 4-aminoazobenzene with adramatic color change only in few minutes.Finally, we successfully applied the concentration of phenthoate pesticides on vegetable, fruit, water, and soil samples. In this method, we found the absorption maxima of the orange dye compound was measuredat480nm.ThecolorsystemobeysBeer’slawinthefollowingworkingrangeof5µg to 40 µg in a final solution of 25 mL. The molar absorptivity, Sandell’s sensitivity, standard deviation, and the relative standard deviation was found to be 1.083X107L mol-1 cm-1, 0.99X10-5µg cm-2, ±0.003 and 0.89% respectively. This method is simple, sensitive, andfree from interferences of other pesticides and diverse ions. Other pesticides do not interfere with the proposed method. The method is highly reproducible and has been successfully applied for the determination of phenthoate insecticides in vegetables and fruit samples.
Cite this article:
Bhatt et al. (2020). Spectrophotometric Determination of Phenthoate in Vegetables and Fruit Samples of Kabirdham (Chhattisgarh). Journal of Ravishankar University (Part-B: Science), 33(1), pp. 08-17.DOI: https://doi.org/10.52228/JRUB.2020-33-1-2
AderonkeAdetutuOkoya, Aderemi Okunola Ogunfowokan, Olabode Idowu Asubiojo, and Nelson Torto (2013), Organochlorine Pesticide Residues in Sediments and Waters from Cocoa Producing Areas of Ondo State, Southwestern Nigeria Hindawi Publishing Corporation ISRN SoilScience.
Alkan, E.; Kapukıran, F.; Er, E.; Chormey, D.; Keyf, S.; Özdoğan, N.; Bakırdere, S.;(2018).,“Simultaneous Determination of Phorate and Oxyfluorfen in Well Water Samples with High Accuracy by GC-MS After Binary Dispersive Liquid-Liquid Microextraction”, Water Air Soil Pollution229-298.
Amin, A.S., Moalla, S.M.N., Ali, A., Salama, M.S., Gouda, A.A.(2015), “SensitiveSpectrophotometric determination of deltamethrin insecticide in its formulation andenvironmental samples”, International Journal of advanced research in chemical science,2, 72-79.
B.S. Choudri, Yassine Charabi, Mushtaque Ahmed (2018), “Pesticides and Herbicides, Water EnvironmentResearch”,Volume90.
Christopher DN, Njoku I, Denis OU, Chinedu A, Grace CO, Paul CE et al (2013).“Investigation on acute toxicity andbehavioral changes in a freshwater African cat fish,Clariasgariepinus exposed to organophosphorouspesticide, Termifos”, Pakistan Journal of Zoology;45:959-965.
J.Chen , C.Chen (2013) , “A new data analysis method to determine pesticide concentrations by cyclic voltammetry , Measurement”,Volume 46, Issue 6, Pages 1828-1833.
Jian Wang,Willis Chow, Daniel Leung, and James Chang (2012)“Application of Ultrahigh-Performance Liquid Chromatography and Electrospray Ionization Quadrupole Orbitrap High-Resolution Mass Spectrometry for Determination of 166 Pesticides in Fruits and Vegetables”,Journal of Agricultural and Food Chemistry;60, 12088−12104.
KarraSomaiah, Satish V.V. Pothula, Baratha Jyothi Naladi and SunitaKanikaram(2015).“Acute toxicity of Phenthoate (50% EC) on survival andbehavioral pattern of freshwater fish Labeorohita(Hamilton, 1822)”,International Journal of Fisheries and Aquatic Studies; 2(6): 38-42.
Kaufmann, A.; Dvorak, V.; Cruzer, C.; Butcher, P.; Maden, K.;Walker, S.; Widmer, M.; Schurmann,( 2012) A. Study of high-resolution massspectrometry technology as a replacement for tandem massspectrometry in the field of quantitative pesticide residue analysis. J.AOAC Int., 95, 528−548.
Khedr, T.; Hammad, A.,; Elmarsafy, A.; Halawa, E.; Soliman, M.; (2019). “Degradation of some organophosphorus pesticides in aqueous solution by gamma irradiation”, Journal of Hazardous Materials.
Kumari, B., Kathpal, T. S., Pesticides: “Methods for their residue estimation”, New
Publishing Agency, 2010, 5.
Margarita Stoytcheva and RoumenZlatev (2014), Organophosphorus Pesticides Analysis, Universidad Autónoma de Baja California Instituto de Ingeniería Mexico.
Nahla S. El-Shenawy,(2014) “Phenthoate induced-oxidative stress in fresh isolated mice hepatocytes: Alleviation by ascorbic acid”. Toxicology and Environmental Health Sciences, Volume 6, Issue2.
Nirmal, M., Rai, M. K., Patel, V., Khatoon,R., Wani, K., Rai, J(2016)., “SensitiveSpectrophotometric Determination of Deltamethrin Using Leuco Malachite Green inEnvironmental”,Asian Journal of chemistry, 28,793-796.
N.M. Bhojane, R.S. Ingole, S.W. Hajare, S.V. Kuralkar, S.P. Waghmare, P.R. Rathod, S.J. Manwar and M.V. Khodke (2018) “Individual and Combined Effect of Indoxacarb and Glyphosate onBiochemical Alterations in Japanese Quails (CuturnixCuturnix Japonica)”Chem Sci Rev Lett, 7(25), 190-200
Notardonato, I.; Russo, M.; Vitali, M.; Protano, C.; Avino, P.; ,(2018). “Analytical Method Validation for Determining Organophosphorus Pesticides in Baby Foods by a Modified Liquid–Liquid Micro extraction Method and Gas Chromatography–Ion Trap/Mass Spectrometry Analysis”, Food Analytical Methods.
Pesticide Residues In Food (1980)Joint meeting of the FAO Panel of Experts on Pesticide Residue in Food and the Environment and the WHO Expert Group on Pesticide Residue Rome, phenthoate.
Pradhan, S., Dhundhel, R.S.,.Rai, M.K, Rai, J.K.(2007), “A new sensitive method for thedetermination of deltamethrin in environmental , agricultutralabnd biologicalsamples”, Journal of the Indian chemical society, 84,1176-1178.
R. Romero-González, A. G. Frenich, and J. L. M. Vidal (2008), “Multi residue method for fast determination of pesticides in fruit juices by ultra-performance liquid chromatography coupled to tandem mass spectrometry”, Talanta, vol. 76, no. 1, pp. 211–225.
Raetano, C.G.Escola Superior de Agricultural Luiz de Queiroz, Piracicaba, SP (Brazil) [Corporate Author] (1991). “Residue determination of phenthoate and malathion by gas chromatography in tomato fruits”.
Reddy, N, D.; Kumar, D.;(2017) “High Pesticide use in India: Health Implication”, Health Action,
Saadati, N., Abdullah, M. P., Zakaria, Z., Sany, S. B. T., Rezayi, M., Hassonizadeh, H.,(2013) “Limit of detection and limit of quantification development procedures fororganochlorine pesticides analysis in water and sediment matrices”, Chemistry Central Journal 7, 63-73..
SomaiahKarra, Satish V.V. Pothula, Baratha Jyothi Naladi and Sunita Kanikaram(2015). “Acute toxicity of Phenthoate (50% EC) on survival and behavioral pattern of fresh water fish Labeorohita” International Journal of Fisheries and Aquatic Studies 2(6):38-42.
Shrivastava, A., Gupta, V. B.,(2011)“Methods for the determination of limit of detection andlimit of quantitation of the analytical methods”, Chronicles of Young Scientists, 2, 1, 21-25.
Susana Grimalt, Pieter Dehouck (2016), “Review of analytical methods for the determination of pesticide residues in grapes”, Journal of Chromatography A, 1433 1–23.
Wu, X.; Li, W.; Guo, P, R.; Zhang, X, Z.; Xu, H, H.; (2018), “Rapid trace detection and isomer quantitation of pesticide residues via Matrix-assisted Laser Desorption/ionization Fourier Transform ion Cyclotron resonance mass spectroscopy”, Journal of agriculture and food chemistry, 66 (15), 3966-3974.
Zhao-YangLi, Zhi-Chao, ZhangQi-Lin, ZhouRu-Yu, GaoQin-Sun Wang,(2002). “Fast and precise determination of phenthoate and its enantiomeric ratio in soil by the matrix solid-phase dispersion method and liquid chromatography” . Volume 977, Issue 1, Pages17-25.