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Author(s): Pushpendra Kumar, Umakant Sahu, Harkesh Dadsena, Lokprabha Hirwani, Chhavi Rahangdale, Abhishek Nand, Bhoomika Swarnkar, Yashika Israni, Narendra Kumar, Vishal Jain

Email(s): pushpendrakumar0311@gmail.com , uksahu28@gmail.com , harkeshdadsena2000@gmail.com , hirwanilokprabha2@gmail.com , chhavirahangdale1236@gmail.com , bhoomikaswarnkar67@gmail.com , abhisheknand008@gmail.com , yashikaisrani2003@gmail.com , narendrakumar.ch1789@gmail.com , vishaljain123@gmail.com

Address: Department of Pharmacognosy, University Institute of Pharmacy, Pt. Ravishankar Shukla University, Raipur, Chhattisgarh, India.
Department of Pharmacognosy, University Institute of Pharmacy, Pt. Ravishankar Shukla University, Raipur, Chhattisgarh, India.
Department of Pharmacognosy, University Institute of Pharmacy, Pt. Ravishankar Shukla University, Raipur, Chhattisgarh, India.
Department of Pharmacognosy, University Institute of Pharmacy, Pt. Ravishankar Shukla University, Raipur, Chhattisgarh, India.
Department of Pharmacognosy, University Institute of Pharmacy, Pt. Ravishankar Shukla University, Raipur, Chhattisgarh, India.
Department of Pharmacognosy, University Institute of Pharmacy, Pt. Ravishankar Shukla University, Raipur, Chhattisgarh, India.
Department of Pharmacognosy, University Institute of Pharmacy, Pt. Ravishankar Shukla University, Raipur, Chhattisgarh, India.
Department of Pharmacognosy, University Institute of Pharmacy, Pt. Ravishankar Shukla University,

Published In:   Volume - 39,      Issue - 1,     Year - 2026

DOI: 10.52228/JRUB.2026-39-1-15  

ABSTRACT:
According to Ayurveda, a harmonious relationship with nature is fundamental for maintaining optimal health. The increasing acceptance and widespread use of herbal remedies highlight the growing relevance of this ancient medical system in enhancing resistance against various diseases. In this context, the present study aims to scientifically validate a classical Ayurvedic formulation, Vasaguduchyadi Kvatha Churna (VGKC), along with its marketed preparation, with its major marker, phenol derivative gallic acid. The work focused on the comprehensive quality evaluation, and standardization was carried out through physicochemical assessments, including extractive values that are soluble in water and alcohol, total ash value, acid insoluble ash value, and loss after drying and preliminary phytochemical screening such as alkaloids, steroids, terpenoids, flavonoids, phenolic content, saponins, and glycosides. High-performance thin-layer chromatography (HPTLC) is an accurate and sensitive method for the identification and quantification of selected marker phenol content, gallic acid, in VGKC and their marketed formulation with a solvent system consisting of toluene: ethyl acetate: methanol: formic acid (4:4:2:0.3 v/v/v). Rf value 0.62 for gallic acid was noted, and it precisely matches the matching bands in Vasaguduchyadi Kvatha Churna. This standardization method has ensured the safety, quality, and consistency of the Vasaguduchyadi Kvatha Churna.

Cite this article:
Kumar, Sahu, Dadsena, Hirwani, Rahangdale, Nand, Swarnkar, Israni, Kumar and Jain (2026). Analytical Standardization of Vasaguduchyadi Kvatha Churna Using Gallic Acid as a Phytomarker. Journal of Ravishankar University (Part-B: Science), 39(1), pp. 247-260. DOI:https://doi.org/10.52228/JRUB.2026-39-1-15


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Anonymous. (2001b). The Ayurvedic Pharmacopeia of India, Part 1 (1st ed., Vol. 1, p. 143). Government of  India, ministry of health and Family welfare, Department of Indian system of  medicine & Homeopathy Appendices 2.2.3. New Delhi: The controller of  publications.

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