Author(s):
Subhadip Banerjee, Ranjit K Harwnsh, Shiv Bahadur, Amit Kar, Pulok Mukherjee
Email(s):
ami.subhadipbanerjee@gmail.com
Address:
School of Natural Product Studies, Department of Pharmaceutical Technology, Jadavpur University, Kolkata - 700 032, India.
Published In:
Volume - 29,
Issue - 1,
Year - 2016
DOI:
Not Available
ABSTRACT:
Trikatu is a well known formulation in Ayurveda and most commonly used from ancient times in India This formulation is consisted of three well known medicinal plants, viz., Piper longum (PL), Piper nigrum (PN) and Zingiber officinale (ZO) in equal ratio. Trikatu has been used for the treatment of cough, cold, fever, asthma, respiratory problems and improvement of digestive disorders. The main objective in the present study was to investigate the effect of individual ingredients of trikatu on drug metabolizing enzymes (CYP3A4 and CYP2D6), to find out its herb-drug interaction potential through cytochrome P450 inhibition assays. Additionally this work was aimed to develop a RP-HPLC method for the identification and quantification of piperine and 6-gingerol in trikatu. Inhibition potential of PL, PN and ZO were studied through CYP450-CO complex assay using rat liver microsomes (RLM) and fluorescence screening method using individual isoenzymes (CYP3A4 and CYP2D6). Quantification of piperine and 6-gingerol in LF, MF and individual plant materials was performed through RP-HPLC method. Inhibitory potential of trikatu formulation in CYP450-CO complex assay were found to be and 35.12 2 2.31% (LF) and against CYP2D6 and CYP3A4 were estimated to be (IC50 251.30 z 3.98 and 245.23 1.92 He/ml) and (IC, 225.50 1.02 and 223.254 + 0.92 ug/mL) respectively. Trikatu formulation and its individual components showed significantly (p < 0.001) less inhibitory activity on individual isoenzymes as compared to the positive control. Outcome of the present study demonstrated that trikatu has less interaction potential with drug metabolizing enzymes.
Cite this article:
Banerjee, Harwnsh, Bahadur, Kar and Mukherjee (2016). Herb-Drug Interaction Potential and RP-HPLC Standardization of Trikatu - An Ayurvedic Formulation. Journal of Ravishankar University (Part-B: Science), 29(1), pp.161-162.