ABSTRACT:
Psoralen is afuranocoumarin used in treatment of skin diseases. In present study, B-CD and its hydrophilic polymeric derivative Epi-B-CD were used to increase the solubility and antioxidant activity of psoralen. Inclusion complex was prepared by kneading method and characterized. Phase solubility study revealed AL type solubility diagram. The increase in solubility of psoralen was greater with Epi-B-CD compared to B-CD. Moreover the antioxidant activity assessed by DPPH method was also increased after complexation with cyclodextrins. The main objective of this study was to evaluate the potential of psoralen-CD complex -loaded nanostructured lipid carriers as a topical delivery system. The NLCS were prepared by emulsion evaporation-solidification method. The average entrapment efficiency and drug loading of the optimized NLCS were 91.1520.13 % and 15.0820.01 %, respectively. Under the transmission electron microscope, the nanoparticles were spherically shaped. The average particle size was 151.2 nm, the zeta potential was -17.0z1.22 mV. Topical delivery of NLCS was investigated in vitro and in vivo. Thus, epichlorohydrin beta cyclodextrin proved to be significant solubilizing and complexing agent with improvement in physicochemical properties of bioactive constituents.