ABSTRACT:
The aim of this study was to develop a potential novel formulation of nanoemulsion containing apigenin using tamarind gum polymeric emulsifier which was having the smallest droplet size, the highest loading, and a good physical stability for Skin delivery, Nanoemulsion containing apigenin have prepared by high specd homogenization and characterized for morphology, zeta potential, differential scanning calorimeter study and penetration studies. In vitro skin permeation of nanoemulsion encapsulated apigenin through goat abdominal skin was determined by the Franz diffusion cell and confocal laser scanning microscope (CLSM). The cytotoxicity of the reported formulation was evaluated in HaCaT Cells (A) and A431 cells (B) by MTT assay. The nanoemulsions were characterized by TEM demonstrated spherical droplets and FTIR to ensure the compatibility among its ingredients. Low fluorescence intensity in CLSM indicated lower cumulative amount of drug permeation from marketed product. CLSM showed uniform fluorescence intensity across the entire depth of skin in nanocarrier treatment, indicating high penetrability of nanoemulsion gel through goat skin. The nanoemulsion gel showed toxicity on melanoma (A341) in a concentration range of 0.4-2.0 mg/ml, but less toxicity toward HaCaT cells by MTT assay. The nanoemulsion gel formulation of apigenin possesses better penetrability across goat skin as compared to marketed formulation. Hence, it can be concluded that novel formulation of apigenin can be proved fruitful for the treatment of skin cancer in near future.