In the present study the nanoprecipitation (or solvent displacement) method was employed to prepare the Chitosan -QUNLS with improved drug incorporation and loading properties. The response surface method with quadratic model was employed to study the effect of selected parameters in the formulation of nanoparticles. The response variable of optimized formulation was fond to be percentage drug entrapment efficiency 51%, 2.89% drug loading capacity and 31.4% drug release. The drug entrapment efficiency of the nanoparticles increases with polymer ratio. Delivery system F, (with Drug:Polymer ratio 1:45), sonication time 15 min. showed maximum drug entrapment efficiency (51%) and Drug loading capacity 2.89% and maximum drug release 31.4% and the mannosylated form of these formulation sahowed 39.1%entrapment efficiency, 1.79% drug loading capacity. These optimized nanocarrier also showed diffusion controlled prolonged release of medicament as indicated shown by in-vitro drug relcase studies. The average size of the optimized formulation were around 112 nm and the average diameter of mannosylated form were 125.7 and zetapotential value -24.4 and -23.7 respectively. The resultant nanoparticles were nearly rough rather than smooth as seen under TEM. All the result provides supplementary evidences that the QUNLS have good entrapment efficiency and drug loading capacity than the mannosylated nanoparticles (M-QUNLS). And on the basis of results we can concluded that the QUNLS and M-QUNLS have good entrapment efficiency, drug loading capacity, mean particle size diameter and zeta potential by the stability point of view and have great targeting and prolonged release effect with great potential in topical delivery.
Cite this article:
Verma and Saraf (2016). Designing of Quercetin Loaded Mannosylated Nanoparticles for Treatment of Skin Cancer. Journal of Ravishankar University (Part-B: Science), 29(1), pp.143-144.