Abstract View

Author(s): Renu Bhatt, Neha Pandey

Email(s): dr.renubhat@yahoo.com

Address: Department of Biotechnology, Guru Ghasidas Vishwavidyalaya, Bilaspur 495 009, India.

Published In:   Volume - 29,      Issue - 1,     Year - 2016

DOI:

ABSTRACT:
Recent developments in the possible use of nanoparticles for carrier of enzyme have attracted great attention. In the present study, the arsenite oxidase (AOase) enzyme capable of transforming the more toxic arsenite (As(III)] to the less toxic arsenate [As(V)] was isolated from arsenic resistant bacteria (Exiguobacterium) and partially purified. Chitosan nanoparticles were prepared on the basis of ionie gelation of chitosan with tripolyphosphate (TPP) anions. The purified AOase was immobilized efficiently by physical adsorption on to chitosan nanoparticles and were characterized for particle size, morphology, zeta potential, AOase loading efficiency and subsequent release kinetics. The chitosan nanoparticles were spherical in shape with the average diameter of 100 nm which increased to 294 nm upon successful loading of AOase. Under optimized conditions, the loading capacity of the chitosan nanoparticle was determined to be 71% for AOase. Further, immobilization also increased the stability of AOase at varying temperature (4-37 "C) and pH (5-10) for a period of 30 days with the increased activity. It also facilitated increased biotransformation of As (III) to As (V) with a potential of five times of reuse. A conceptual understanding of biological responses to AOase loaded chitosan nanoparticle is needed for the development of novel methods of drug delivery.

Cite this article:
Not Available


References not available.

Related Images:



Recent Images



Modeling of Abnormal Hysteresis in CsPbBr3 based Perovskite Solar Cells
Impact of Melatonin on Growth and Antioxidant Activity of Cicer arietinum L. Grown under Arsenic Stress
Assessment of Cymoxanil in Soil, Water and Vegetable Samples
Various Techniques of MPPT Based Charge Controller & Comparison of A/C with D/C Home Appliances - A Review
Need of Gallium Recovery from Waste Samples: A Review
Higher Order Statistics Based Blind Steg analysis using Deep Learning
Covid-19 related School Closure Impact on School going Children & Adolescents of Raipur, Chhattisgarh
Determination of Pentachlorophenol in Environmental Samples by Spectrophotometry
Simple and Cost Effective Polymer Modified Gold Nanoparticles Based on Colorimetric Determination of L-Cysteine in Food Samples
Investigation on Design and Device Modeling of High Performance CH3NH3PbI3-xClx Perovskite Solar Cells

Tags


Recomonded Articles: