ABSTRACT:
Nowday's liver disorders due to exposure of different types of drug/chemical molecules are common. The objective of the present study was to evaluate the protective effects of Sharbat-e-Deenar (SD) against acetaminophen (APAP, paracetamol) induced liver damage. The present study was divided into three parts, including (1) measurement of free radical scavenging and choleretic activity, (2) evaluating of effective dose of SD (1, 2, 4 ml/kg, p.o.) against APAP exposure (2 g/kg. pa) for acute study and (3) confirmation of selected dose of SD for hepatoprotection against subchronic exposure of APAP (20 mg/kg. po, for 20 days) in rats, Blood parameters, tissue biochemical parameters, comet assay and histological observations were performed. APAP administration in rats induced a significant rise in biochemical parameters such as aspartate aminotransaminase (AST), alanine aminotransaminase (ALT), lacatate dehydrogenase (LDH), and serum alkaline phosphatase (SALP). These biochemical changes were inhibited by treatment with SD at all doses as compared to control group. Treatment of rats with APAP at the dose of 20 mg/kg led to an increase in the level of lipid peroxidation and significant reduction of the oxidative markers such as reduced glutathione, catalase, superoxide dismutase, glutathione peroxidase, glutathione reductase and microsomal aniline hydroxylase. SD treatment significantly restored the APAP- induced alterations in the biochemical and oxidative stress markers of liver. The hepatoprotective effect of SD was also confirmed by the histopathological examination and comet assay of liver tissue. These data suggest that the Shabat-e-Deenar may act as a hepatoprotective and antioxidant agent.