The sorpotion-desorptio behavior of arginine (Arg) and histidine (His) from 90% ethanol on HClO4/CH3COOH- treated alumina of surface phase pH 3.0-9.0 is described. The elution behaviour and column chromatography were also investigated to correlate the data. The sorption of Arg and His was found to depend on the pH of the substrate as well as on the acid used for pretreatment of the oxide. The sorption was found to Increase in the pH range 4.0-9.0. The process was moderately fast (between 10 min and 24 hr) and almost athermic (20°-50°C). Desorption efficacy and retarding influence on adsorption by cations was in the order: Th4+ > Al3+ > Mg2+ > Na+,The distribution coefficient for edsorption (Kd) and desorption (KD) were determined, and on the basis of these studies, the elution behaviour and column chromatography was carried out.
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Cite this article:
Kaur, Mundhara, Tiwari and Kar (1991). Studies on the Sorption Behaviour of the Amino Acids, Arginine and Histidine on Chemically-Pretreated Alumina in relation to Chromatography. Journal of Ravishankar University (Part-B: Science), 4(1), pp.47-59.