ABSTRACT:
Chemical pretreatment of hydrous oxides as a means of activation of ion-exchangeable sites has been advocated. Amino acids are ampholytes and change of pH beyond' their isotectric point solution has a significant election the net-charge, which in turn. can play an important role in their adsorption behaviour on polar adsorbents like alumina. To investigate the concept further, sorption desorption behavior of the amino acids glycine• and serine from 90% ethanol on HClO4 and•CH3COOH- treated alumina of surface phase pH 3:0. - 9.0 was investigated. The sorption was found to depend on pH of the substrate surface as well as the acid used for pretreatment Maximum sorption was. obs•erved at pH 5.0 to 7.0. However, it decreased in the pH range•5.0, 3.0 and 7.0 to 9.0. except for serine on HCI04 treated alumina. where increasing adsorption was found in the pH range 4.0-9.0. The process was moderately fast (1Omin-24hr) and almost athermic (20° - 50°C). Desorp.tion efficacy and retarding influence on the adsorption by eteotrolytes was in the order Th (N03)4 >Al (N03)3 >•Mg (N03)2 > CH3COONa > NaN03.
It appears that the controlling force tor adsorption is predominantly ion- exchange. The distribution coefficients for adsorption (Kd. Cm3 g-1) and desorption (Kd, g cm-3) were determined by batch operation, and on the basis of these parameters, the elution behavior of the solutes was investigated. The data elution behaviour of the solutes was investigated. The data were further employed in their column chromatography.
Cite this article:
Kaur, Mundhara, Tiwari, and Kar (1989). Sorption desorption studies of the amino acid, glycine and serine on chemically pretreated alumina with relation to chromatography. Journal of Ravishankar University (Part-B: Science), 2(1), pp. 23-35.