Abstract View

Author(s): Sandhya Chandrakar, AK Gupta

Email(s): sandhyachandrakar86@gmail.com

Address: School of Studies in Life Science, Pt. Ravishankar Shukla University, Raipur 492 010, India.

Published In:   Volume - 29,      Issue - 1,     Year - 2016

DOI: Not Available

ABSTRACT:
Endophytic actinomycetes are becoming popular for their bioactive molecules and are relatively unexplored in Chhattisgarh. In the present investigation, a total of nine endophytic Streptomyces sp. were isolated from Abutilon indicum (L.) sweet through surface sterilization process. Isolate K-RI showed maximum antibacterial activity against Gram-positive human pathogens B. cereus MTCC-430, B. subtilis MTCC-441, S. aureus MTCC-96, S. epidermidis MTCC-435 and clinical human pathogens B. cereus JNMC-1, B. subtilis JNMC-2 and S. epidermidis JNMC-4. Based on morphology, biochemical and physiological properties using PIB WIN software the isolate K-RI was identified as Streptomyces phaeochromogenes with ID score of 0.97. Ten different basal production medium were assessed for evaluation of a suitable medium for antibiotic production. Glucose soybean meal broth medium showed a maximum antibacterial activity and was used for further optimization studies. The optimized culture condition for antibacterial compound production using central composite design was as: Inoculum size-3.9 %, Incubation time-222 h, pH-7.62, Temperature-30.25°C. In addition, optimization enhanced the antibacterial activity spectrum also. The compound was extracted with ethyl acetate and tested for purity by thin-layer chromatography. A single spot with Rf value of 0.66 was detected. Further purification using HPLC and characterization of the active molecule is at hand.

Cite this article:
Chandrakar and Gupta (2016). Antibacterial Compound from Endophytic Streptomyces sp (K-RI) from Root of Abutilon indicum (L) Sweet. Journal of Ravishankar University (Part-B: Science), 29(1), pp.193-194.


References not available.

Related Images:



Recent Images



Potential of Bioactive Compounds for Atopic Dermatitis
Time-Frequency Image-based Speech Emotion Recognition using Artificial Neural Network
Zn Fortification Influential Impact on the Productivity of Calocybe indica Mycelium
Enhanced ACO in clustering algorithm for QoS in WSN enabled IoT
A Modified Regression Type Estimator Using Two Auxiliary Variables
Sziklai Pair based Small Signal Amplifier with BJT-MOSFET Hybrid Unit at 180nm Technology
Rice Straw-Derived Carbon Integrated with PANI: As an Electrode Material for High-performance Supercapacitor
Extraction, Characterisation, Biological Properties and Applications of Essential Oils: A Review
Biopolymeric Materials in the Management of Diabetic Wound Healing: A Comprehensive Review
Entomopathogenic Fungi: Nature

Tags


Recomonded Articles:

Author(s): Swati Jain; Somesh Kumar Dewangan

DOI: 10.52228/JRUB.2021-34-1-9         Access: Open Access Read More

Author(s): Pooja Deshpande, Suman Shrivastava, S.J. Daharwal*

DOI: 10.52228/JRUB.2018-31-1-7         Access: Open Access Read More

Author(s): Anil Kumar Verma*; Swati Sahu; Mohan Patel; Sanjay Tiwari

DOI: 10.52228/JRUB.2020-33-1-5         Access: Open Access Read More

Author(s): AR Sood and RC Rathor

DOI:         Access: Open Access Read More

Author(s): Subhendu Saha

DOI:         Access: Open Access Read More

Author(s): Dinesh Kumar Sharma; Bhabani S Nayak

DOI:         Access: Open Access Read More

Author(s): B S Ajitkumar; Prashant S Hande; Vikas Jha; Pratiksha Alagh; Shailendra Rane

DOI:         Access: Open Access Read More

Author(s): Sayan Biswas; Joydeb Chanda; Amrendra Tiwari; Pulok K Mukherjee

DOI:         Access: Open Access Read More

Author(s): Swapnil Deshmukh; Nikhil Chawala; Atharv Bhide; Shekhar Waikar

DOI:         Access: Open Access Read More

Author(s): Shiv Shankar Shukla; Ravindra Pandey; Swarnalata Saraf; Shailendra Saraf

DOI:         Access: Open Access Read More

Author(s): Minakshi Waghmare; Kalyani Kale; Deepti Bhagchandani; Shekhar Waikar

DOI:         Access: Open Access Read More

Author(s): Yogyata Chawre; Lakshita Dewangan; Ankita Beena Kujur; Indrapal Karbhal; Rekha Nagwanshi; Vishal Jain; Manmohan L. Satnami

DOI: 10.52228/JRUB.2022-35-1-7         Access: Open Access Read More

Author(s): Roshan Shahu; Bhavana Hirudkar; Shekhar Waikar; Arti Shanware

DOI:         Access: Open Access Read More

Author(s): Mohammad A Rashid

DOI:         Access: Open Access Read More