Endophytic actinomycetes are becoming popular for their bioactive molecules and are relatively unexplored in Chhattisgarh. In the present investigation, a total of nine endophytic Streptomyces sp. were isolated from Abutilon indicum (L.) sweet through surface sterilization process. Isolate K-RI showed maximum antibacterial activity against Gram-positive human pathogens B. cereus MTCC-430, B. subtilis MTCC-441, S. aureus MTCC-96, S. epidermidis MTCC-435 and clinical human pathogens B. cereus JNMC-1, B. subtilis JNMC-2 and S. epidermidis JNMC-4. Based on morphology, biochemical and physiological properties using PIB WIN software the isolate K-RI was identified as Streptomyces phaeochromogenes with ID score of 0.97. Ten different basal production medium were assessed for evaluation of a suitable medium for antibiotic production. Glucose soybean meal broth medium showed a maximum antibacterial activity and was used for further optimization studies. The optimized culture condition for antibacterial compound production using central composite design was as: Inoculum size-3.9 %, Incubation time-222 h, pH-7.62, Temperature-30.25°C. In addition, optimization enhanced the antibacterial activity spectrum also. The compound was extracted with ethyl acetate and tested for purity by thin-layer chromatography. A single spot with Rf value of 0.66 was detected. Further purification using HPLC and characterization of the active molecule is at hand.
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Cite this article:
Chandrakar and Gupta (2016). Antibacterial Compound from Endophytic Streptomyces sp (K-RI) from Root of Abutilon indicum (L) Sweet. Journal of Ravishankar University (Part-B: Science), 29(1), pp.193-194.