At present, medical world is struggling with the resistance developed in microbial pathogens against currently available antibiotics. Hence there is a great necessity of novel antibiotics which can destroy the microbial pathogens. Traditional medicinal plants are rich source of antimicrobial compounds. In this context research on various extracts of Ocimum sanctum leaves have shown significant results. Ocimum sanctum plants from surrounding area of Rajim Dist. Gariyaband Chhattisgarh were selected for this study. Leaves stem and root of Ocimum sanctum plants were first dried in shade at room temperature then grounded to fine powder and stored at room temperature. Extracts were taken by three methods viz methanol extracts, ethanolic extracts and aqueous extract. Extracts from all the three types of extraction process showed antibacterial activity against Gram positive bacteria like Streptococcus, Bacillus subtilis and gram negative bacteria like Escherichia coli. Leaves of Ocimum sanctum showed higher antibacterial activity in low concentration compare to stem and root extracts. Methanol extracts showed significantly higher antibacterial activity than extracts from other two types of extraction process. Minimum inhibitory concentration data showed hopeful result as some of the extracts exhibited significant inhibition of bacteria even at low concentration. The zone of inhibition was measured in millimeter using vernier caliper. This study indicate that leaves have significant higher antibacterial activity as compared to stem and root of Ocimum sanctum and it could be very useful for developing a novel antibacterial agents.
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Cite this article:
Sahu and Gawri (2016). A Comparative Study of Antibacterial Activity against Some Bacterial Stain between Different Parts of Ocimum Sanctum Tulsi. Journal of Ravishankar University (Part-B: Science), 29(1), pp.164-165.