Abstract View

Author(s): Deepika Mahobiya, A.K. Gupta

Email(s): deepikamahobiya@gmail.com

Address: School of Studies in Life Science, Pt. Ravishankar Shukla University, Raipur - 492 010, Chhattisgarh, India

Published In:   Volume - 28,      Issue - 1,     Year - 2015

ABSTRACT:
L-asparaginase is a chemotherapeutic agent that degrades L-asparagine present in blood and indirectly starve tumour cell which lead to cell death. Endophytic fungi from medicinal plants are known to produce pharmaceutical rortant metabolites and enzymes. In the present investigation, eighty endophytic fungi were isolated from six medicinal he viz. Aloe vera (L.) Burm. f., Abrus precatorius L., Asparagus racemosus Willd., Catharanthus roseus (L.) G. Don., Hemidesmus indicus (L.) R. Br., Plumbago zeylanica L. and three medicinal shrubs, viz., Abutilon indicum (L.) Sweet, Adhatoda vasica Nees and Ocimum sanctum L. Eleven genera of fungi including Acremonium, Alternaria, Aspergillus, Aureobasidium, Bipolaris, Colletotrichum, Curvularia, Fusarium, Paecilomyces, Penicillium, Pestalotiopsis, and seven etivity sterile forms were identified. Out of eighty isolated endophytic fungi, forty four isolates showed L-asparaginase activity through plate assay, while nineteen isolates exhibited L-asparaginase activity upon submerged fermentation. Notably, Fusarium species isolated from the roots of A. precatorius, A. vasica, A. vera, C. roseus and H. indicus exhibited promising L-asparaginase activity. Collerotrichum gloeosporioides isolated from the stem of A. racemosus and C. roseus also exhibited good L-asparaginase activity. Fusarium solani (Mart.) Sacc. NFCCI-3301(GR6) showed maximum L-asparaginase and selected for optimization purpose. Modified Czapek Dox medium was selected as the best production medium among the cight media. Maximum L-asparaginase activity was recorded with 1.0% L-asparagine on third day of fermentation in the modified Czapek Dox medium. Soyabean meal was found to be the best additional source of nitrogen which increased production by two fold with F, solani. Potassium nitrate was the second best source of nitrogen. Addition of different carbon source in place of glucose did not affect L-asparaginase production.

Cite this article:
Mahobiya and Gupta (2015). Endophytic Fungi from Selected Herbs & Shrubs of Raipur and their L-asparaginase Producing Potentials. Journal of Ravishankar University (Part-B: Science), 28(1), pp. 51-59.


References not available.

Related Images:



Recent Images



Performance Evaluation of Spectrogram Based Epilepsy Detection Techniques Using Gray Scale Features
Perovskite Solar Cells an Efficient, Low Cost, Emerging Photovoltaic Technology
Spectrophotometric Determination of Phenthoate in Vegetables and Fruit Samples of Kabirdham (Chhattisgarh)
Flotation-Dissolution-Spectrophotometric Determination of Phorate in Various Environmental Samples
Preparation, Fabrication and Characterization of Sol-Gel ZnO Thin Films for Organic Solar Cells
Distribution of Some Selected Surface Active Agents (SAAs) in the Aquatic and Global Environment with Their Toxic Impact: A Comprehensive Review
Intriguing Clinical and Pharmaceutical Applications of IERs: A Mini Review
Soil Contamination in the Industrial Vicinity of Bemetara and Raipur District of Chhattisgarh, India
An Extractive Spectrophotometric Method for the Determination of Pymetrozine in Various Ecological Samples of Bilaspur District (C.G.)
Development and Characterization of Quercetin Loaded Nanoparticle for Skin Cancer

Tags


Recomonded Articles:

Author(s): Suman Shrivastava, Pooja Deshpande, S. J. Daharwal*

DOI:         Access: Open Access Read More

Author(s): Monika Jain; S.K. Chatterjee; Manoj Jangde; Deepak Sina*

DOI:         Access: Open Access Read More

Author(s): Anushree Saha; Manas Kanti Deb*; Mithlesh Mahilang; Shubhra Sinha

DOI:         Access: Open Access Read More

Author(s): Subhalakshmi Ghosh; Banasri Hazra

DOI:         Access: Open Access Read More

Author(s): Swati Sahu; Rajesh Awasthy; Mohan Patel; Anil Verma; Sanjay Tiwari

DOI:         Access: Open Access Read More

Author(s): Deepika Mahobiya; A.K. Gupta

DOI:         Access: Open Access Read More

Author(s): R.C. Maurya;A.K. Sharma;P.K. Vishwkarma;J.M. Mir;B.A. Malik;D.K. Rajak

DOI:         Access: Open Access Read More

Author(s): Prasad A Wadegaonkar

DOI:         Access: Open Access Read More

Author(s): Rajib Bandyopadhyay

DOI:         Access: Open Access Read More

Author(s): C K Katiyar

DOI:         Access: Open Access Read More

Author(s): Sanmoy Karmakar

DOI:         Access: Open Access Read More

Author(s): Deepika Mahobiya; A K Gupta

DOI:         Access: Open Access Read More

Author(s): Suchita Agrawal; Prabha Rohatgi

DOI:         Access: Open Access Read More

Author(s): Deependra Singh; Manju R Singh; A N Bahadur

DOI:         Access: Open Access Read More