L-asparaginase is a chemotherapeutic agent that degrades L-asparagine present in blood and indirectly starve tumour cell which lead to cell death. Endophytic fungi from medicinal plants are known to produce pharmaceutical rortant metabolites and enzymes. In the present investigation, eighty endophytic fungi were isolated from six medicinal he viz. Aloe vera (L.) Burm. f., Abrus precatorius L., Asparagus racemosus Willd., Catharanthus roseus (L.) G. Don., Hemidesmus indicus (L.) R. Br., Plumbago zeylanica L. and three medicinal shrubs, viz., Abutilon indicum (L.) Sweet, Adhatoda vasica Nees and Ocimum sanctum L. Eleven genera of fungi including Acremonium, Alternaria, Aspergillus, Aureobasidium, Bipolaris, Colletotrichum, Curvularia, Fusarium, Paecilomyces, Penicillium, Pestalotiopsis, and seven etivity sterile forms were identified. Out of eighty isolated endophytic fungi, forty four isolates showed L-asparaginase activity through plate assay, while nineteen isolates exhibited L-asparaginase activity upon submerged fermentation. Notably, Fusarium species isolated from the roots of A. precatorius, A. vasica, A. vera, C. roseus and H. indicus exhibited promising L-asparaginase activity. Collerotrichum gloeosporioides isolated from the stem of A. racemosus and C. roseus also exhibited good L-asparaginase activity. Fusarium solani (Mart.) Sacc. NFCCI-3301(GR6) showed maximum L-asparaginase and selected for optimization purpose. Modified Czapek Dox medium was selected as the best production medium among the cight media. Maximum L-asparaginase activity was recorded with 1.0% L-asparagine on third day of fermentation in the modified Czapek Dox medium. Soyabean meal was found to be the best additional source of nitrogen which increased production by two fold with F, solani. Potassium nitrate was the second best source of nitrogen. Addition of different carbon source in place of glucose did not affect L-asparaginase production.
Cite this article:
Mahobiya and Gupta (2015). Endophytic Fungi from Selected Herbs & Shrubs of Raipur and their L-asparaginase Producing Potentials. Journal of Ravishankar University (Part-B: Science), 28(1), pp. 51-59.