ABSTRACT:
Lantana camara, commonly known as wild sage, causes hepatotoxicity in grazing animals, characterized by intrahepatic cholestasis, liver damage and photosensitization. The present experimental study was undertaken to evaluate the comparative ameliorating effectiveness of four herbal plants, including Gingko biloba, Berberis lycium, Tinospora cordifolia, and Hippophae salicifolia against lantadene-induced sub-acute toxicity, which mimics the conditions of lantana toxicity in grazing cattle. The sub-acute toxic dose of lantadenes, 25 mg/kg bw was used for inducing toxicity in guinea pigs. A total of 44 guinea pigs of either sex were divided into 7 groups. Among these, 4 groups receiving herbal extracts were further sub- divided into 2 sub-groups with 4 animals in each group. Sub-groups of each herbal extract treated group received plant extract at the dose of 100 and 200 mg/kg bw, respectively. One group received silymarin which served as positive control. oral of the ameliorating plants was extract The started a week prior to the administration of lantadenes. The total duration of the trial was 21 days, The results of gross pathology, histopathology, apoptosis detection, serum marker enzyme values and oxidation stress levels suggested that G. biloba followed by B. lycium, T. cordifolia and H. salicifolia in decreasing order were effective in preventing lantadenes- induced damage in a dose-dependent manner. The results of apoptosis detection in liver tissue showed that lantadenes at the dose rate of 25 mg/kg bw were able to cause cell death both by apoptosis as well as necrosis. freeze-dried methanolic of the administration.