Currently available synthetic anti-inflammatory drugs pose a major problem during their clinical uses. Therefore, efforts are being made by several researchers to develop novel herbal based anti-inflammatory drugs. Many plant species known in folk medicine of different cultures used for their anti-inflammatory and analgesic properties were proved to be good for the treatment of inflammatory conditions and pain. The present study was aimed to identify the anti-Inflammatory activity of leaves of Lagascamollis (Cav.) family Asteraceae. The active ingredient was extracted by continuous hot extraction method using ethanol as solvent. The extract was then lyophilized and the dried material was stored in a desiccator. The extract was then subjected for different pharmacognostic, phytochemical studies and different constituents like flavonoids, tannins were estimated quantitatively. Male Wistar rats (250-260g) were chosen as a pharmacological model to study the anti-inflammatory activity of the extract.In first series of studies; acute oral toxicity study was performed. The extract caused no mortality in rats and the extracts were found to be safe upto a dose of 2000 mg/kg/bw. Based on toxicity study data, two doses of 200and 400mg/kg/bw were selected for examining the biological activity. Carrageenan and Egg albumin were selected as noxious agents to induce paw edema in rats. Theethanolic, aqueous, ethyl acetate and n-butanol extracts shows significant reduction in inflammation Further, as compared with standard drug diclofenac (15 mg/kg), the effect was slightly better. Among all the extracts the ethanolic extract at a dose of 400 mg/kg maximum suppression of paw edema after 120 minutes.In necropsy study, the injury at the site of administration was not registered. Viewed collectively. the results of the present study showed that ethanolic extract of the leaves of Lagascamollis exhibit potent anti-inflammatory activity without producingany untoward effects.
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Cite this article:
Agrawal and Sahu (2016). Extraction, Characterization and in-vivo Screening of Anti-inflammatory Activities of Lagas camollis cav. Journal of Ravishankar University (Part-B: Science), 29(1), pp.198-199.