The present study evaluated the antihypertrophic potential of the ethanolic extract of Boerhaavia diffusa (BDE), a well- known edible cardiotonic plant reported in Ayurveda against angiotensin Il- induced hypertrophy in H9c2 cardiac myoblast cell. Markers of hypertrophy such as cell size, protein content and the concentrations of atrial natriuretic peptide (ANP) and B-type natriuretic peptide (BNP) were analysed for the confirmation of hypertrophy induction. Angiotensin I1 (100nm) caused an increase in cell volume (69.26 (SD 1.21)%), protein content (48.48(SD 1.64)%), ANP (81.90 (SD 1.22)%) and BNP (108.57 (SD 1.47)%). BDE treatment significantly reduced cell volume, protein content and the concentrations of ANP and BNP (P0.05) in H9c2 cells. The activity of various antioxidant enzymes and the concentration of reduced glutathione, which was lowered duc to hypertrophy, were increased in BDE-treated cells. The BDE treatment also reduced lipid peroxidation and protein carbonyls in cells. The mitochondrial dysfunction during hypertrophy was evident from increased intracellular ROS production (26.2520.91%), mitochondrial superoxide generation (65.06±2.27%), alteration in mitochondrial transmembrane potential (AYm), opening of mitochondrial permeability transition pore (MPTP) and mitochondrial swelling. In addition, activities of mitochondrial respiratory chain complexes (I-IV), aconitase, NADPH oxidase, thioredoxin reductase, and oxygen consumption rate and calcium homeostasis were found altered. Treatment with BDE significantly prevented the generation of intracellular ROS and protected mitochondrial function and integrity. Overall results revealed the protective effects of B. diffusa against hypertrophy in H9c2 cells and the present findings may shed new light on the therapeutic potential of B. diffusa in addition to its nutraceutical potentials.
Cite this article:
Raghu (2016). Protective Effect of Boerhaavia diffusa L against Angiotensin II Induced Hypertrophy in H9c2 Cells. Journal of Ravishankar University (Part-B: Science), 29(1), pp.191-192.