Abstract View

Author(s): V Badireenath Konkimalla

Email(s): badireenath@niser.ac.in

Address: NISER, Bhuhaneswar - 75100, Orissa.

Published In:   Volume - 29,      Issue - 1,     Year - 2016

DOI:

ABSTRACT:
The cure rates in cancer chemotherapy are affected by the development of drug resistance and severe side effects. Due to these limitations, there is an urgent need for improved therapeutics. Bioactive compounds from medicinal plants represent a valuable resource for novel anticancer drugs. To gain a systematic approach, we have established a library of more than 2400 cytotoxic natural products derived from traditional Chinese medicine. A total of 531 compounds revealed considerable cytotoxicity against 60 cancer cell lines of the National Cancer Institute (NCI), USA. The IC50 values of 60 cell lines for these 531 natural products were correlated with the microarray-based mRNA expression of 95 genes involved in metabolism and signalling of nitric oxide (NO), in order to identify candidate compounds as NO inhibitors. The cut-off value for the Pearson's correlation analysis was R>0.6. The best correlation was found between the mRNA expression of TXNRDI (thioredoxin reductase 1) and bis(helenaliny)glutarate. Experimental validation of this in silico approach was performed with NO measurement using RAW264.7 mouse macrophages. Indeed, we showed that bis(helenalinyl)glutarate at concentrations of 2 or 4ug/ml strongly inhibited NO generation. As a next step, the 95 genes related to NO metabolism and signalling were analyzed for their association with this compound, in order to elucidate underlying signalling pathways. In conclusion, approaches using natural compound libraries and in silico screening techniques are suitable to identify novel NO inhibitors as shown with bischelenaliny) glutarate.

Cite this article:
Not Available


References not available.

Related Images:



Recent Images



Herbal Alternatives for Oral Health:  Mechanistic Exploration with their Market Potential
Time of the Day Variability in Pit-Building Behavior of Antlion Larvae
A Comprehensive Review of a particular Skin Injury: Pathogenesis, triggers, and current Treatment Options
Enhanced antioxidant activity in Curcuma caesia Roxb. microrhizomes treated with silver nanoparticles
Studies on the Interaction of Imidazolium Ionic Liquids with Human Serum Albumin
Basic and Advanced Logical Concept Derived from Surface Enhanced Infrared Spectroscopy (SEIRS) as Sensing Probe for Analysis of Chemical Species: A Brief Review
Soil Erosion Risk Estimation by using Semi Empirical RUSLE model: A case study of Maniyari Basin, Chhattisgarh
An Estimator of Population Variance Using Multi-Auxiliary Information
Microemulsion as Novel Drug Delivery for Fungal Eye Infection
Potential of Bioactive Compounds for Atopic Dermatitis

Tags


Recomonded Articles: