Abstract View

Author(s): Prasoon Gupta

Email(s): guptap@iim.ac.in

Address: Department of Natural Product Chemistry, Indian Institute of Integrative Medicine, Canal Road, Jammu-180001, India.

Published In:   Volume - 29,      Issue - 1,     Year - 2016


Human embryonic stem cells (HESCS) are a unique cell type isolated from the inner cell mass of pre-implantation blastocysts'. Theoretically, HESCS are capable of unlimited self-renewal and can differentiate to every somatic cell type in the body. In addition to that they may provide a system to study early human development in vitro, and in the future, differentiated populations can be used for cell replacement therapies. The advent of defined media and controlled differentiation has enabled screening of hESCs to discover molecules that impact growth, differentiation or apoptosis in undifferentiated and differentiating populations. Small molecules such as natural products could be highly valuable in the progression of cell therapy technologies, or could be novel therapeutics in their own right, providing tools for embryonic cell populations that cannot be targeted with other approaches. In our ongoing research program of screening pre-fractionated and. semi-purified extracts of plants in an effort to discover compounds that impact hESCs growth using a 96-well plate real-time cell electronic sensing (RT-CES) system, activity was discovered for the extract of the Magnolia grandiflora. A large-scale extraction and purification yielded two new diterpenoids (1-2), along with known compounds (3-6). The cell growth inhibitory activities of compounds 1-6 were evaluated against HESCS (BG02) and a pancreatic cell line (BXPC-3) using an RT-CES system. Compounds (1) showed the greatest growth inhibition against both the BG02 and BXPC-3 cells with ECS0 values of 2.4 and 9.3 pM, respectively.

Cite this article:
Not Available

References not available.

Related Images:

Recent Images

Performance Evaluation of Spectrogram Based Epilepsy Detection Techniques Using Gray Scale Features
Perovskite Solar Cells an Efficient, Low Cost, Emerging Photovoltaic Technology
Spectrophotometric Determination of Phenthoate in Vegetables and Fruit Samples of Kabirdham (Chhattisgarh)
Flotation-Dissolution-Spectrophotometric Determination of Phorate in Various Environmental Samples
Preparation, Fabrication and Characterization of Sol-Gel ZnO Thin Films for Organic Solar Cells
Distribution of Some Selected Surface Active Agents (SAAs) in the Aquatic and Global Environment with Their Toxic Impact: A Comprehensive Review
Intriguing Clinical and Pharmaceutical Applications of IERs: A Mini Review
Soil Contamination in the Industrial Vicinity of Bemetara and Raipur District of Chhattisgarh, India
An Extractive Spectrophotometric Method for the Determination of Pymetrozine in Various Ecological Samples of Bilaspur District (C.G.)
Development and Characterization of Quercetin Loaded Nanoparticle for Skin Cancer


Recomonded Articles: