Abstract View

Author(s): Ravishankar Chauhan, Afaque Quraishi, S K Jadhav, S Keshavkant

Email(s): ravi_9br@gmail.com

Address: School of Studies in Biotechnology, Pt. Ravishankar Shukla University, Raipur 492 010.

Published In:   Volume - 29,      Issue - 1,     Year - 2016

DOI:

ABSTRACT:
Safed Musli (Chlorophytum borivilianum) is one of the most commercially important medicinal herbs of India. Present investigation reports on vitrification based cryopreservation of in vitro-grown meristematic dome of C. borivilianum. The in vitro regenerated stock cultures were preconditioned on culture medium with different concentration of sucrose for 8 weeks. Then the meristematic regions of culture were excised and exposed to growth retardants for 24 h. These pre-cultured meristematic domes were again trimmed up to 3-5 mm length and treated with chilled PVS2 solution. These domes were kept in cryo-vials of 5 ml filled with PVS2 solution and then directly plunged into LN, for 1 day. These cryopreserved samples were subjected to rapid re-warm at 38-40°C via water bath for three min. The cryopreserved samples were post-cultured in the dark for three days and then transferred to normal photoperiod regime of 16:8. The mean regeneration rate of cultures was amounted to approx 25% after cryopreservation. Plants developed from cryopreserved samples were morphologically normal.

Cite this article:
Not Available


References not available.

Related Images:



Recent Images



Study of the Enhanced Efficiency of Crystalline Silicon Solar Cells by Optimizing Anti Reflecting Coating using PC1D Simulation
Formulation of Topical Itraconazole Nanostructured Lipid Carriers (Nlc) Gel for Onychomycosis
Cosmetic Testing Equipment: Device and Types of Equipment for Dermatological Evaluation for Women’s Skin
Oxidative stress: Insights into the Pathogenesis and Treatment of Alopecia
UV Spectroscopy Analysis for Itraconazole
Challenges and Potential of Perovskite Solar Cells
Kinetic Study of Solvent Effect on the Hydrolysis of  Mono-3, 5-Dimethylaniline Phosphate
OLED: New Generation Display Technology
Parametric study of AlGAN/GaN UV-Led Based on Quantum Confined Stark Effect (QCSE)
Analysis of High Efficient Perovskite Solar Cells Using Machine Learning

Tags


Recomonded Articles: