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Author(s): Ravishankar Chauhan, Afaque Quraishi, S K Jadhav, Keshav Kant Sahu

Email(s): ravi_9bt@ymail.com

Address: School of Studies in Biotechnology Pt. Ravishankar Shukla University, Raipur 492 010.

Published In:   Volume - 29,      Issue - 1,     Year - 2016

DOI:

ABSTRACT:
The present investigation deals with micro-tuberization and slow growth strategies for in vitro conservation of Chlorophytum borivilianum (Safed Musli) as it is categorized as a rare herb of India with economic importance. The experiment was divided in two phases; first one in vitro tuber production and second conservation via slow growth of the cultures. MS medium supplemented with different concentration of source was tested for micro-tuber induction. Sucrose and commercial sugar were examined for slow growth studies. A higher sucrose level was found suitable for large number of invitro tuber formation. For storage of the cultures, much higher cconcentrations of carbohydrate were found suitable ; and the cultures were successfully stored for four months with 100% survival and the cultures were found morphologically normal when transferred to the normal shoot proliferation medium, after the storage.

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