ABSTRACT:
Tinospora cordifolia is being used from a long time for the treatment of several diseases in Indian system of medicine. In the present study the inhibition potential of Tinospora cordifolia extracts and its constituent tinosporaside to cause herb-drug interactions through rat and human liver cytochrome enzymes was evaluated. Further metals content was estimated through atomic absorption spectroscopy. Bioactive compound was quantified through RP-HPLC, in order to standardize the plant extract. Interaction potential of the test samples were evaluated by CYP450-carbonmonoxide complex (CYP450-CO) assay with pooled rat liver microsome. Influence on individual recombinant human liver microsomes such as CYPJA4, CYP2D6, CYP2C9 and CYPIA2 isozymes were analyzed through fluorescence microplate screening assay and respective IC values were determined. The content of tinosporaside was found to be 1.64% (w/w) in Tinospora cordifolia extract. Concentration dependent inhibition was observed through Tinospora cordifolia extract. Observed IC (ug/ml) value were 136 45 (CYPJA4), 144.37 (CYP2D6). 127.55 (CYP2C9) and 141.82 (CYPIA2). Tinosporaside and extract showed higher IC (ug/ml) value than the known inhibitors. Heavy metals were found to be within the prescribed limits as per WHO and US-FDA guidelines. Plant extract showed significantly higher IC value than respective positive inhibitors against CYPJA4, 2D6, 2C9 and IA2 isozymes. Present study concluded that consumption of Tinospora cordifolia may not cause any adverse effects when consumed along with other xenobiotics.