Glory lily (Gloriosa superba L.) is an herbaceous climber and globally interested important medicinal plant, due to the presence of active ingredient colchicine. G. superba is distributed in tropical parts of India. It possesses a strong apical dominance character in nature, which is a notable reason for pushing it in threaten category. The present study focused on development of microtuberization technique for conservation and propagation of Glory lily. The meristematic regions of underground tubers were selected as explants. The in vitro study was carried on MS medium containing different combination of plant growth regulator (PGR) and other adjuvant such as citric acid, Polyvinyl pyrrolidone-40 and sucrose. Maximum numbers of microtubers were obtained in the presence of BA and containing higher level of sucrose in the culture medium. The regenerated microtubers were produced shoots; they were get separated and subjected to in vitro hardening. The in vitro hardened plantlets with functional roots and photo synthetically active leaves were successfully transferred to green house with 95 % survival.
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