ABSTRACT:
The plant Bridelia retusa spreng belonging to the family Euphorbiaceae is commonly known as Patharphod, khaja has long been used in traditional system of medicine as astringent, anthelmintic and in many skin infections. The bark with gingili oil is much used as liniment in rheumatism. Several compounds including flavonoids, lignans, polyphenols, alkaloids, triterpenes were isolated from these plants & some of them interacts with most of the key enzymes. Various pharmacological studies were carried out with the extracts & purified compounds. The survey of literature has revealed that the flavonoid rich fraction of the plant has not been investigated for the anti-microbiabacterial activity. The acerial parts of B. retusawere collected from the local Nagpur region. It was identified by the Department of Botany, RTM Nagpur University, Nagpur with the help of authentic herbarium species. In the present investigationsthe material was dried in shade and then coarsely powdered and was used for the extraction of total flavonoid fraction. The dried acrial part was defatted with the help of petroleum ether (60-80") & the marc was used to isolate flavonoid rich fraction. The flavonoid rich fraction was evaluated qualitatively for the presence of flavonoid with the help of preliminary Phytochemical test and Thin Layer Chromatography. The fraction was then evaluated for the anti-microbial activity with the help of Gram-positive, Gram- negative bacteria and Fungi.