ABSTRACT:
The present study was focused to evaluate the possible interaction potential of standardized extract of Coriendrum sativum (CS) on drug metabolizing enzymes through CYP450 inhibition study. CS is used as food and also in therapy in Indian Systems of Medicine. CS and its bioactive compounds were studied for evaluation of inhibition potential on pooled CYP450, as well as human CYP3A4 and CYP2D6. The plant extract was standardized through RP-HPLC (Waters 600, C column) using linalool as marker. Methanol: Water (80:20 v/v) with 1% Acetic acid was optimized as mobile phase and elute was detected at 210 nm. The presence of heavy metal (Pb, Cd, As & Hg) in the medicinal plant was determined by Atomic Absorption Spectroscopy. Interaction potential of the standardized CS extract and phytoconstituent with pooled CYP450 were significantly less (p<0.05: pc0.01: P<0.001) than standard known inhibitor. In the present study, extract showed higher inhibition than their single bioactive compound. The concentrations of heavy metals present in the plant extract were within the permissible limit. From the above study, we can conclude that the CS and linalool contributed negligible food-drug interactions for the tested isozymes and may not possess any harmful effect upon their therapeutic benefits.