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Author(s): Roshan Shahu, Bhavana Hirudkar, Shekhar Waikar, Arti Shanware

Email(s): roshan2116@ gmail.com

Address: Gurunanak College of Pharmacy, Deepak Nagar, Behind CP Foundary, Nari Road, Nagpur - 440 026, India
Rajiv Gandhi Biotechnology Center, Laxminarayan Institute of Technology, Amravati Road, Nagpur - 440 026, India.

Published In:   Volume - 29,      Issue - 1,     Year - 2016

DOI:

ABSTRACT:
The plants of the genus Phyllanthus belonging to the family Euphorbiaceae, are widely distributed in most tropical and sub-tropical countries, and have long been used the folk medicine to treat diabetes, hepatitis B, hepatitis C, urinary bladder disturbances & kidney disturbances. Several compounds including flavonoids, lignans, polyphenols, alkaloids, triterpenes were isolated from these plants & some of them interacts with most of the key enzymes. Various pharmacological studies were carried out with the extracts & purified compounds of the genus Phyllanthus and these studies supports most of their reported uses in folk medicine as antiviral agents, antinociceptive agents and in the treatment of genitourinary disorder. In India many Phyllanthus species have long been used in the treatment of hepatitis, diabetes and inflammatory disorders. P. mederaspatensis occurs abundantly in cultivated paddy fields in the local Nagpur region. The survey of literature has revealed that the flavonoid rich fraction of the plant has not been investigated for the anti-microbial activity. The acerial parts of P. mederaspatensis were collected from the local Nagpur region. It was identified by the Department of Botany, RTM Nagpur University, Nagpur with the help of authentic herbarium species. In the present investigations the material was dried in shade and then coarsely powdered and was used for the extraction of total flavonoid fraction. The dried acrial part was defatted with the help of petroleum ether (60-80") & the marc was used to isolate flavonoid rich fraction. The flavonoid rich fraction was evaluated qualitatively for the presence of flavonoid with the help of preliminary phytochemical test and thin layer chromatography. The fraction was then evaluated for the anti-microbial activity with the help of Gram-positive, Gram- negative bacteria and Fungi.

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