ABSTRACT:
Earlier in vivo studies on rabbits and rats reported that the administration of sodium salicylate, an active component of analgesic, antipyretic and anti-inflammatory drugs like aspirin and paracetamol, induces toxic effects on liver and erythrocytes due to reactive oxygen species generated due to this drug. The present study was planned under in vitro condition to confirm these effects, and evaluate antioxidant potential of medicinal plants (Withania Somnifera, Curculigo orchioides and Tinospora cordifolia) in salicylate treated erythrocytes considering changes in lipid peroxide (LPO) of membrane of erythrocytes in series of in vitro assay. Lipid peroxidation (LPO) in erythrocytes was determined in terms of malonyldialdehyde (MDA) formed as a measure of oxidation of polyunsaturated lipids. It was quantified by measuring malonyldialdehyde (MDA) produced using thiobarbituric acid (TBA) by the method of Stocks and Dormandy (1971). The result showed that there was significant (P<0.001) increase in LPO activity of erythrocytes exposed to sodium salicylate at 10, 20 and 30 ppm concentration. Treatment with Withania Somnifera herb extract at different concentration significantly (P0.001) lowers LPO activity. In case of Curculigo orchioides and Tinospora cordifolia herb extracts LPO activity was not significantly alters at lower concentration of extract but significantly alters at higher concentration. It is concluded that the herb extract has strong antioxidant property, which scavenges reactive oxygen species (ROS) generated by sodium salicylate treatment in erythrocytes of goat and these extracts could be a potential sources of natural antioxidant that could have great important as therapeutic agents.