Bioethanol is considered as an important renewable fuel to partly replace fossil-derived fuels. Fermentation of sugar-based raw materials is refered to as first generation bioethanol, whereas the use of lignocellulosic raw materials is commonly called second generation bioethanol. The third generation of algal bioethanol is at an early stage of investigation. Bioethanol is the most valuable bio energy source which produced by the fermentation of carbohydrate containing substrates using various microorganisms. Present study deals with the bioethanol production from sal seeds and comparative analysis of bioethanol production by two different yeasts Saccharomyces cerevisiae MTCC 4780 and Pichia kudriavzevii. Different parameters temperature and pH were also optimized. It was observed that both yeasts produced maximum bioethanol at 30 Cand pH 5. In comparison to Saccharomyces cerevisiae, Pichia kudriavzevii was more supported the production of biocthanol.
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