Hairy root cultures are induced from Agrobacterium rhizogenes mediated transformation technique. Transgenic hairy root is an optimistic source of secondary metabolites in medicinal plant with high pharmaceutical value and for the production of phytochemicals or medicinally important enzymes and foreign proteins either in in-vitro conditions or through plant regeneration. The hairy roots are grown in vitro in bioreactors to study their soil interaction with other pathogens like fungi, nematodes and to study the mass production of various secondary metabolites. So, this technique is a powerful tool in the field of ethno pharmacology. The study emphasized on the development of a reliable and well defined protocol for the induction of hairy root through Agrobacterium rhizogene mediated transformation in Vigna radiata. Vigna radiata can be used as antioxidant in treating various conditions like liver disease, cancer, diabetes, kidney disorders and for curing various microbial infections. This process is divided into three phases: first maintenance of Agrobacterium rhizogene (MTCC 532) culture in different culture medium like Nutrient Broth (NB), Yeast Extract Peptone (YEP) and Yeast Mannitol Broth (YMB), second was in-vitro germination of different seeds has been observed, Vigna radiata was showed highest rate of germination and third was initiation of hairy root culture in different Vigna radiata 2nd day seedlings was showed 70% transformed roots.
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