Present work was designed to perform phytochemical screening, estimate total phenolics, flavonoids content and antiotidant activity of Pueraria tuberosa tubers. The dried powder of Pueraria uberosa tubers was extracted exhaustively by soshlet with ethanol and fractionated with hexane, ethyl acetate, and water. All the prepared extracts were analyzed to identify the chemical compounds present in the crude extracts. Folin-Ciocalteu reagent and colorimetric methods were used to estimate total phenolic and flavonoid content in the extracts. In- vitro antioxidant activity was determined by DPPH method. Phytochemical analysis of ethanolic extract showed the presence of major classes of phytochemicals. Ethanolic extract and ethyl acetate fraction was found to contain highest phenolic content and flavonoid. In-vitro antioxidant activity of ethanol extract and ethyl acetate fraction was significant (pc0.05) and comparable with the standard ascorbic acid. Result of the study shows that tuber of Pueraria tuberosa are rich source of phenolic.and flavonoid compounds that can play an important role in preventing the progression of many discases. These finding provide evidence that the extract of Pueraria fuberose is a natural source of antioxidant.
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