ABSTRACT:
To progress, cancers require a source of nutrition and oxygen. Tumour that lack angiogenesis remain doman rapid logarithmic growth follows the acquisition of a blood supply, tumour angiogenesis triggers this activation. Neoplain are able to synthesize or induce certain polypeptides, VEGF is one of the most critical factors that induce angiogenesis d being targeted for anti-angiogenesis treatment. However, most of current anti-VEGF agents cause side effects when given chronically, need of naturally ocurring VEGF inhibitors is highly evident. In present study a phenolic fraction of ethanolic extract of Mulberry fruit was subjected to study in- vitro anti-kinase activity and chick aortic ring assay. ELISA assay kit was used to determine the ability of MBE (mulberry extract) to inhibit VEGFR2 tyrosine kinase activity. A strong inhibition of VEGFR kinase activity, with an ICo of about 10ng/ml was exhibited. In aortic ring assay, chick aortic rings were embedded in the matrigel and fed with medium containing different concentrations of MBE, rings were then stimulated with VEGF or ECGS and sprout formation was observed microscopically, a dose dependent decrease in capillary sprouting was observed with MBE treatments, the growing sprouts were shorter and fewer cells migrated into the matrix indicating that MBE could inhibit VEGF and ECGS-induced micro vessel sprouting. This study warrants the potential usefulness of MBE as a safe, natural VEGF inhibitor.