ABSTRACT:
The present study was evaluated to bring out the diversity of thcnnotolerant bacteria isolated from river Shivnath, vhich is a major source of water for Durg-Bhilai region. Membrane filtration was performed for isolating bacteria from water ud filter was incubated at 44.5°C for isolating thermotolerant bacteria. Total of I 3 bacterial isolates viz. Escherichia coli ,z=.5), Salmonella sp, (1), Vibrio sp. (1), Pseudomonas fluorescence (4), Klebsiella sp. (2) were identified using biochemical nd morphological analysis. Antibiotic resistance pattern performed for gentamycin, streptomycin, nalidixic acid, arnpicillin, .iloramphenicol, co-trimoxazole, ceftriaxone and tetracycline. The highest number of isolates (69.23%) was resistant to xlidixie acid, followed by co-trirnoxazole (61.5%) and ampieillin (53.8%). Least resistance was observed for streptomycin 1%) and chloramphenicol (23%). Pseudomonas was found to be sensitive to all antibiotics. Multidrug resistance was shown y Pseudomonas sp., Vibrio sp., Klebsiella sp., E. coli. Plasmid profiling was performed for the presence of resistance bands .ir all isolates. Presence of high molecular weight plasmid bands was noted in all isolates except I E. coli isolate, indicating ·=l.romosome mediated resistance.