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Author(s): Seema A Belorkar, AK Gupta, Vibhuti Rai

Email(s): seema.belorkar@gmail.com

Address: Department of Microbiology and Bioinformatics, Bilaspur University, Bilaspur - 495 006, India
Sos in Lifesciences, Pt.Ravishankar Shukla University, Raipur 492 010, India
Ex-Head, SoS in Lifesciences, Pt. Ravishankar Shukla University, Raipur -492 010, India.

Published In:   Volume - 29,      Issue - 1,     Year - 2016

DOI:

ABSTRACT:
Fructooligosaccharides are now well known for their prebiotic properties. Moreover, they lower cholesterol levels, phospholipids and triglycerides in the blood, as well as the diastolic blood pressure. Their synthesis is now commonly accomplished by microbial fructosyltransferase (Flase) enzyme which has gained parallel importance in food market. The present study intends to maximize the Ftase production of A. stallus by amending the nutritional, physical and cultivation conditions of the mold. The investigation of effect of physical parameters like incubation period, pH and temperature revealed the optimum conditions to be day 4 at pH 6.00 and 30'C incubation temperature. Sucrose was the best utilized carbon source for Ftase production at 5 % concentration and supported 36.05 0.10 IU/ml of enzyme Beef extract was optimally utilized at 2% as nitrogen source enhancing Ftase production to 36.05 + 0.10 IU/ml. The study of additives revealed 123 33% increase in Ftase production due to MBSO, The introduction of shaking conditions increased the Ftase production to 68.73 2 0.10 IU/ml. The total enhancement of Ftase production was 201.44%.

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