Antioxidant prevents oxidative stress or damage of macromolecules such as carbohydrate, proteins, DNA, lipid etc. due to free radicals. Most of the medicinal plants posses antionidant properties imparted by a lot of secondary metabolites. In the present study, antioxidant activity of different concentration (Sul,10ul,20ul) of methanolic extract of flower of Sphagneticola trilobata was evaluated in H,O; induced lymphocytes (10,000 cells) of Oryctolagus cuniculus L by assessing the change in concentration of MDA,GSH,SOD.CAT and GPx and compared the results with standard and established antioxidant, Melatonin, in 8 groups (I- only lymphocytes, II- lymphocytes+100 uM HO, II- lymphocytes+100 M HO, +Sul PLE, IV. lymphocytes+100 uM H;O, +10ul PLE,V. lymphocytes+ 100 uM HO, +20ul PLE, VI- lymphocytes+100 uM H,O, +Sul Melatonin, VII- lymphocytes+100 uM HO, +10ul Melatonin, VIII- lymphocytes+100 uM HO, +20ul Melatonin). We found the activity of all antioxidant enzymes (SOD, CAT, GSH, GPx) except MDA were significantly increased in plant extract treated group (i.e.2.5320.10, 3.0320.25, 3.420.8, 6.60:0.10 respectively) with 20 ul of extract but still it was found less effective as compared to melatonin treated group (3.16:0.11, 4.70:0.03,5.7+0.4, 9.640.06 respectively). MDA which is indication of lipid peroxidation was found significantly decreased in plant extract treated group (4.0:0 39) but still it was found less effective as compared to melatonin treated group (1.30.19). From this finding, we can suggest that the flower of this plant possesses antioxidant property and further research is required for identification of specific plant compound which can be applicable for pharmacological usage.
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