Cite this article:
Mishra and Achari (2017). Transformation of Rana tigrina during Progressive Metamorphosis. Journal of Ravishankar University (Part-B: Science), 30(1), pp.102-109.
Journal of Ravishankar
University-B, 30 (I &II), 102-109 (2017)
Transformation
of Rana tigrina during Progressive Metamorphosis
Dhananjay Mishra and K.Venu
Achari
Department of Zoology, Govt.
E. R. R. PG Science College, Bilaspur - 495006, India
Department of Zoology, Govt.
College Sanawal, Dist. Balrampur - 497220, India
Corresponding author: kvenu99@rediffmail.com
[Received 17 September 2015; accepted 2 April 2016]
Abstract: We determined the kinetics
of metamorphosis, apoptosis, and tail regression in Rana tigrina. Acid
phosphatase activity (µMole Pi.hr-1.tail-1) in the growing and regressing tail
attended six to thirty fold increase respectively. However total activity in
the trunk was decreased through progressive growing stages of metamorphosis. Total
protein content in the trunk of tadpoles at climax stage (XXI) was decrease
(35%) from 2.6mg/ml to 1.7mg/ml. The tail of tadpole tissue has shown a two
fold increase in total Ribonucleic Acid (RNA) content from stage III to stage
XVIII. But there was again decrease in total RNA content at climax stage (stage
XXI). This might be possible due to decreased protein synthetic status. When
the experiment was performed in trunk homogenate the amount of total carbohydrate
(mg/ml) was slightly increased from 37mg/ml to 38.6mg/ml. this might be due to
increase in the activity of α-amylase enzymes in the viscera of developing
tadpole when it reached the climax stage.
Keywords: Rana tigrina,
metamorphosis, apoptosis, acid phosphatase, ribonucleic acid
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