Cite this article:
Singh and Daharwal (2016). Development of Classical Least Square Method for the Determination of Ambroxol hydrochloride and Guaifenesin in Cough Syrup. Journal of Ravishankar University (Part-B: Science), 29(2), pp.29-34.
Journal of Ravishankar University - B,
29 (2&3) pp.29-34 (2016)
Development of Classical Least Square Method for the
Determination of Ambroxol hydrochloride and Guaifenesin in Cough Syrup
Veena D.Singh and S.J.
Daharwal
University
institute of Pharmacy, Pt. Ravishankar Shukla University, Raipur-402010,
Chhattisgarh, India
Corresponding
author: veena1806@gmail.com
[Received
13 October 2015; revised version 30 September 2016; accepted 28 August 2016]
Abstract :
A new simple spectrophotometric method was developed for the determination of
binary mixtures of Ambroxol hydrochloride (AMB) and Guaifenesin (GUF), without
prior separation. The method is based on the multi-wavelength technique i.e.
classical least square (CLS) method. The liquid formulation of Ambroxol
hydrochloride and Guaifenesin was determined by classical least square at 244
of AMB and at 225 and 272 nm of GUF. Beer's law was obeyed in 5-50 µg/ml of
each drug and calibration equation was obtained. The obtained result was shown
that the mean recoveries were more than 98% for AMB and GUF, respectively. The
proposed spectrophotometric method was validated and successfully applied for
the assay of drug combination in several laboratory-prepared mixtures and
commercial pharmaceutical formulations.
Keywords: Spectrophotometric analysis, Classical
least square method, Ambroxol hydrochloride, Guaifenesin, Method validation
NOTE: Full version of this manuscript is available
in PDF.