Author(s):
Mahesh Kumar Kharat, Milind Patole, Kalpana Pai
Email(s):
kalpanapai@wnipune.ac.in
Address:
Department of Zoology, Savitribai Phule Pune University, Ganeshkhind, Pune - 411 007, Maharashtra, India
National Centre for Cell Science, NCCCS Complex, University of Pune Campus, Ganeshkhind, Pune 411 007, Maharashtra, India.
Published In:
Volume - 29,
Issue - 1,
Year - 2016
Cite this article:
Kharat, Patole and Pai (2016). In Vitro Antileishmanial Activity of the Medicinal Plant Extract of Aerva sanguinolenta (Linn) Blume. Journal of Ravishankar University (Part-B: Science), 29(1), pp.98-99.
OP-F03
In Vitro
Antileishmanial Activity of the Medicinal Plant Extract of Aerva sanguinolenta
(Linn) Blume
Mahesh Kumar Kharat, Milind
Patole and Kalpana Pai
Department of Zoology, Savitribai
Phule Pune University, Ganeshkhind, Pune - 411 007, Maharashtra, India
National Centre for Cell Science,
NCCCS Complex, University of Pune Campus, Ganeshkhind, Pune 411 007,
Maharashtra, India
Corresponding author email: kalpanapai@wnipune.ac.in
[Received
01 January 2016; accepted 15 January 2016]
Abstract: Leishmania
donovani causative agent for visceral leishmaniasis is transmitted to humans by
sandfly vector. It is caused by an obligate intra-macrophage protozoan parasite
Leishmania. It accounts for high morbidity and mortality. There are two phases
of development in Leishmania parasites (i) intra-macrophage 'amastigote form
and (i) in sandfly as promastigote' form. Therapeutic drugs in clinic are
pentavalent Antimonials, Amphotericin-B (Parentral drug) and Miltefosine.
Vaccines are still not available for leishmaniasis. Focus on control measures
are therefore directed on vector control or more appropriately chemotherapy.
Proper targets are selected, such as an enzyme, through screening of the
biochemical influence of compounds (potential drugs) on this target. However,
their severe toxicity to normal cells and the high resistance of the causative
agent, L donovani, has necessitated the need for discovery of new alternative
therapy. In the present study, we have evaluated Aerva sanguinolenta for
anti-leishmanial potential using L donovani promastigotes (Dd8, WHO Strain). A.
sanguinolenta is an ornamental plant which is used to cure various diseases and
skin lesions. The whole plant ethanolic extract from Aerva sanguinolenta was
investigated for anti-leishmanial activity. A. sanguinolenta showed significant
anti-leishmanial activity as observed by significant ICS0 levels and decrease
in acid phosphatase, arginase, and protein levels as compared with medium alone
and Amphotericin-B. It increased nitric oxide levels, which is indicative of
oxidative stress in parasites. Present study shows that A. sanguinolenta is a
good anti-leishmanial agent. Further studies are warranted to check parasite
clearance in an intra-macrophage model.
Keywords: Aerva
sanguinolenta, Kala-azar, Leishmania, Cytotoxicity, Intracellular protozoan