Cite this article:
Keshavkant, Tandon and Chandra (2016). Responses of Cicer arietinum L. Radicles Towards Different Rates of Drying. Journal of Ravishankar University (Part-B: Science), 29(1), pp.90-91.
OP-D11
Responses
of Cicer arietinum L. Radicles Towards Different Rates of Drying
S
Keshavkant, Mona Tandon and Jipsi Chandra
School
of Studies in Biotechnology, P. Ravishankar Shukla University, Raipur 492 010,
India
Corresponding
author email: skeshavkant@gmail.com
[Received
21 January 2016, accepted 27 January 2016]
Abstract:
Orthodox seed serves as popular model to investigate desiccation-sensitivity in
plant tissues because once they undergo germination, become susceptible towards
desiccation induced damages. In the study undertaken, impacts of rate of
desiccation on the viability status, leakage of cellular electrolytes, content
of superoxide anion radical, lipid-protein oxidation and activities of defense
enzymes were monitored in early seedlings of Cicer arietinum L. under
desiccation and wet storage. For both the drying conditions, desiccation could
be explained by exponential and inverse functions. Under rapid desiccation
tissue viability as measured by germination efficiency and tetrazolium staining
remained 100% all through the analysis (24 h) but declined remarkably (P <
0.05) after 0.30 g g' fresh mass water content (4 days) under slow drying.
Moreover, considerable decline (P < 0.05) in tissue viability was observed
after two weeks of wet storage. Rapid drying was also accompanied with limited
amounts of electrolyte leakage, superoxide anion radical, malondialdehyde and
protein hydroperoxide, together with enhanced content of protein. Additionally,
activities of both superoxide dismutase and ascorbate peroxidase were increased
in rapidly dried radicles, but guaiacol peroxidase was declined. In contrary,
above referred biomarkers were observed to perform either inversely or poorly
during slow drying and wet storage suggesting that above documented alterations
might be the resultant of ageing and not desiccation. Gathered data
demonstrated that increased drying lowers the critical water content for tissue
survival and also reduces the risk of damage resulting from aqueous-based
deleterious reactions. Additionally, it also showed that growing radicles are a
popular model to explore desiccation- sensitivity in plant tissues and/or
seeds.
Keywords:
Antioxidants, Cicer arietinum L., Desiccation sensitivity, Lipid peroxidation,
Reactive oxygen species, Water content