Cite this article:
Harwansh, Mukherje, Biswas, Biswas and Bahadur (2016). Nanoemulsion Based Nano-Gel ofr Genistien Enhancing UVA Protection Efficacy and Bioavailability by Improving its Pharmacokinetics through Transdermal Delivery. Journal of Ravishankar University (Part-B: Science), 29(1), pp.88-89.
OP-D08
Nanoemulsion
Based Nano-Gel ofr Genistien Enhancing UVA Protection Efficacy and
Bioavailability by Improving its Pharmacokinetics through Transdermal Delivery
Ranjit
K Harwansh, Pulok K Mukherje, Rajarshi Biswas, Sayan Biswas and Shiv Bahadur
School
of Natural Product Studies, Department of Pharmaceutical Technology, Jadavpur
University, Kolkata-700032, India
Corresponding
author email: harwanshranjeer@gmail.com
[Received
18 January 2016 accepted 29 January 2016]
Abstract:
The study was aimed to develop a genistien (GN) loaded nanoemulsion based
nano-gel in order to ensure increased photo-protection efficacy against UVA
induced oxidative stress and its enhanced bioavailability through transdermal
delivery. The optimized GN-NE2 composed of an appropriate ratio (15:30.55 %
w/w) of the oil (labrafac Lipophile WL1349), water and Smix (surfactant
(labrasol) and co-surfactant (PEG 400)] respectively. GN-NE2 was characterized
by through droplet size, zeta potential, refractive index, TEM, UV and FTIR
analysis. Ex vivo skin permeation and in vivo UVA protection activity of GN-NE2
based nano-gels (GN-NG2) along with placebo-NG were studied through the rat
skin. The pharkacokinetics and bioavailability of pure GN and its nano-gel,
GN-NG2 was studied at dose of 20 mg/kg in rats. GN-NG2 exhibited enhanced UVA
protection efficacy and antioxidant potential due to its improved
pharmacokinetics (Cmat: 95.06 z 4.09 ngml"; tuzel: 3.81 2 0.25 h) and %
relative bioavailability (352.58) when compared to pure GN. Nano- gel
significantly ('P < 0.01) released the drug in a sustained manner to
maintain the minimum effective concentration for extended period of times.
Nano-gel could elevate the level of skin marker enzymes against oxidative stress
mediated by UVA. Thus, transdermal route of nano-gel of GN offers an effective
strategy for enhancing their bioavailability.
Keywords:
Nanoemulsion; Genistien, Bioavailability: Oxidative stress; UVA protection
activity, Nano-gel