Cite this article:
Panik, Singh and Singh (2016). Preparation and Characterization of PLGA Nanoparticle: Formulation and Optimization through Entrapment Efficiency, Drug Loading and Particle Size. Journal of Ravishankar University (Part-B: Science), 29(1), pp.76-77.
OP-C04
Preparation and
Characterization of PLGA Nanoparticle: Formulation and Optimization through
Entrapment Efficiency, Drug Loading and Particle Size
Rajni Kant Panik, Manju Singh and
Deependra Singh
University Institute of Pharmacy,
Pr. Ravishankar Shukla University, Raipur - 492 010, Chhattisgarh
Corresponding author email: panik.143@gmail.com
[Received
5 January 2016; accepted 13 January 2016]
Abstract: Present work
was based with objective to optimize formulations of mupirocin loaded PLGA
nanocarriers using response surface methodology (RSM). The miscellaneous design
model (two-factor three level factorial design) was used for optimization of
formulations was considered for optimization. There were three parameters, drug
entrapment efficiency (EE), drug loading (DL) percentage, and mean particle
size of drug loaded nanocarrier, considered for investigating the optimal
formulation with respect to two independent variables, including drug
concentration (XI) and polymer concentration (X2). The result showed that the
optimal nanocarrier was composed of drug concentration(XI) 10mg and polymer
concentration (X2) 20 mg with %EE of 85.46 t 4.01%, DL of 30.2520.76, mean
particle size of 120.24554 nm, polydispersity index (PDI) of 0.20320.067, and
zeta potential value of -8.672(-4.45) mV. DSC and TEM study showed that there
was no chemical interaction between mupirocin and polymer (PLGA) and the
mupirocin- PLGA nanoparticle are non spherical, respectively. The drug release
experiments exhibited a sustained release over during 24 h, up to 60.26 z
2.83%. Accelerated stability studies showed that there was no significant
change occurring in the responses after storage condition for a total period of
3 months.
Keywords: Nanoparticle,
Entrapment, Nanocarrier