Cite this article:
Sah and Suresh (2016). Penetration Study of Loteprednol Etabonate Loaded Polymeric Nanoparticle across the Excise Goat Cornea: A Study with Confocal Microscopy. Journal of Ravishankar University (Part-B: Science), 29(1), pp.74-75.
OP-C01
Penetration
Study of Loteprednol Etabonate Loaded Polymeric Nanoparticle across the Excise
Goat Cornea: A Study with Confocal Microscopy
Abhishek K Sah and Preeti K
Suresh
University Institute of Pharmacy,
Pt. Ravishankar Shukla University. Raipur 492 010, India
Corresponding author email: abhisheksah9@gmail.com
[Received
30 December 2015; accepted 13 January 2016]
Abstract: The aim of
this research work was to investigate the interaction of loteprednol etabonate
(LE) loaded poly (D, L- lactide co-glycolide) (PLGA) polymer based
nanoparticles with ocular mucosa ex vivo. Rhodamine (Rd) was used as a
fluorescent marker compound to prepare Rd-LE-PLGA-NPs. The formulation was
characterized for various parameters like particle size, polydispersity index
(PDI), zeta potential, XRD, DSC, surface morphology, drug entrapment and
ex-vivo permeation profile. The prepared formulation was evaluated for their
penetration profile in freshly excised goat cornea using Franz diffusion
apparatus and confocal laser scanning microscopy (CLSM). The integrity of the
comeal tissue was investigated by optical digital microscopy of the
histopathological slide stained with hematoxylene and eosin dye. The result
clearly showed that drug loaded nanoparticle exhibited better permeation
profile as compared to plain drug suspension. Additionally, less intensity of
fluorescence was observed from drug suspension as compared to LE-PLGA-NPs.
There was uniform and intense florescence observed across the total depth of
excised goat corneal tissue, suggesting improved penetration profile of
nanoparticles, which can be attributed to the nano size range of the
formulation and precorneal retent of the nanocarrier. Ex vivo permeation showed
prolonged release pattern from NPs for up to 4 hours. The entrapment efficiency
and mean diameter was found to be 96.3121.68 % and 167.620.37 nm respectively.
The TEM confirmed the nano dimension of the particles. The results advocate
that the prepared novel nanoparticulates could be a potential carrier for
ophthalmic therapeutics.
Keywords: CLSM,
Loteprednol etabonate, Nanoparticle, Ocular delivery. PLGA