Cite this article:
Chauhan, Quraishi, Jadhav and Keshavkant (2016). In vitro Conservation of Chlorophytum borivilianum Sant et Fernand, via Vitrification Based Cryopreservation. Journal of Ravishankar University (Part-B: Science), 29(1), pp.73.https://doi.org/
OP-B04
In vitro
Conservation of Chlorophytum borivilianum Sant et Fernand, via Vitrification
Based Cryopreservation
Ravishankar Chauhan, Afaque
Quraishi, SK Jadhav and S Keshavkant
School of Studies in
Biotechnology, Pt. Ravishankar Shukla University, Raipur 492 010
Corresponding author email: ravi_9br@gmail.com
[Received
30 December 2016; accepted 15 January 2016]
Abstract: Safed Musli
(Chlorophytum borivilianum) is one of the most commercially important medicinal
herbs of India. Present investigation reports on vitrification based
cryopreservation of in vitro-grown meristematic dome of C. borivilianum. The in
vitro regenerated stock cultures were preconditioned on culture medium with
different concentration of sucrose for 8 weeks. Then the meristematic regions
of culture were excised and exposed to growth retardants for 24 h. These
pre-cultured meristematic domes were again trimmed up to 3-5 mm length and
treated with chilled PVS2 solution. These domes were kept in cryo-vials of 5 ml
filled with PVS2 solution and then directly plunged into LN, for 1 day. These
cryopreserved samples were subjected to rapid re-warm at 38-40°C via water bath
for three min. The cryopreserved samples were post-cultured in the dark for
three days and then transferred to normal photoperiod regime of 16:8. The mean
regeneration rate of cultures was amounted to approx 25% after
cryopreservation. Plants developed from cryopreserved samples were
morphologically normal.
Keywords: In vitro,
Growth retardants, Meristematic dome, PVS2, Vitrification