Cite this article:
Chauhan, Quraishi, Jadhav and Sahu (2016). In vitro Tuberization and Slow Growth Conservation of Chlorophytum borivilianum Sant et Fernand. Journal of Ravishankar University (Part-B: Science), 29(1), pp.73.
OP-B03
In vitro
Tuberization and Slow Growth Conservation of Chlorophytum borivilianum Sant et
Fernand
Ravishankar Chauhan, Afaque
Quraishi, SK Jadhav and Keshav Kant Sahu
School of Studies in
Biotechnology Pt. Ravishankar Shukla University, Raipur 492 010
Corresponding author email: ravi_9bt@ymail.com
[Received
5 January 2016; accepted 12 January 2016]
Abstract: The present
investigation deals with micro-tuberization and slow growth strategies for in
vitro conservation of Chlorophytum borivilianum (Safed Musli) as it is
categorized as a rare herb of India with economic importance. The experiment
was divided in two phases; first one in vitro tuber production and second
conservation via slow growth of the cultures. MS medium supplemented with
different concentration of source was tested for micro-tuber induction. Sucrose
and commercial sugar were examined for slow growth studies. A higher sucrose
level was found suitable for large number of invitro tuber formation. For storage
of the cultures, much higher cconcentrations of carbohydrate were found
suitable ; and the cultures were successfully stored for four months with 100%
survival and the cultures were found morphologically normal when transferred to
the normal shoot proliferation medium, after the storage.
Keyword: Carbohydrate
source, In vitro conservation, Micro-tuber, Slow growth, Sourose.