Cite this article:
Kumar and Thakur (2016). A Bio-Ethnopharmacology Approach: Development and Characterization of Chitosan-Alginate Polymeric Formulation for Oral Insulin Administration. Journal of Ravishankar University (Part-B: Science), 29(1), pp.70-71.
OP-A10
A
Bio-Ethnopharmacology Approach: Development and Characterization of
Chitosan-Alginate Polymeric Formulation for Oral Insulin Administration
Awanish Kumar and Parul Thakur
Department of Biotechnology, NIT
Raipur, Chhattisgarh, India
Oils Oleochemicals &
Surfactant Technology, ICT Mumbai, India
Corresponding author email:
parulthakur507@gmail.com
[Received
18 January 2016; accepted 28 January 2016]
Abstract: Nature is a
huge enigma, having an inexhaustible plate of mysteries to fill man's insatiable
thirst of knowledge Two such products of nature, alginate and chitosan, have
potential to be used in wide variety of encapsulated drug formulations.
Diabetes Mellitus is a multi-factorial and multi-consequential chronic
metabolic disorder with the hallmark feature of hyperglycaemia. Current therapy
through oral anti-diabetic drugs and subcutaneous administration of insulin
suffers from serious disadvantages of occasional hypoglycaemia due to
unmonitored dosage and lipoatrophy. These approaches don't mimic normal
physiological pattern of insulin release. Thus, bio-compatible oral insulin
formulations, if made possible, would prove to be a boon. This preliminary
study included 100IU insulin encapsulated in 1% CS (in 5% acetic acid) + 2% ALG
(in 2% CaCl). Insulin encapsulated CS ALG beads were prepared by simple
extrusion method. At pH 74 (intestinal), swelling ratio of coated beads is
significantly higher indicating that this formulation can be used for
intestinal peptide drug delivery. SEM gave an average size of 30-50mm.Also,
shift of characteristic bond peaks in FTIR spectra of CS/ALGINS loaded beads is
indicative of insulin encapsulation. Nitrogen in EDX results validates the
interaction between chitosan and alginate, hence, the blending. Release rate graph
suggests, in 9hrs maximum cumulative release achieved was near to 20%.
Entrapment Efficiency of beads was calculated as 39%. Therefore, release was
about 50%. The best linearity, with R'-0.85, was found in Higuchi's equation
plot indicating the release of drug from matrix as a square root of time
dependent process based on Fickian diffusion. Korsmeyer Peppas model,
n>0.89, suggest release mechanism to be Super Case II Transport.
Keywords: Diabetes
mellitus, Oral insulin