Cite this article:
Agrawal and Sahu (2016). Extraction, Characterization and in-vivo Screening of Anti-inflammatory Activities of Lagas camollis cav. Journal of Ravishankar University (Part-B: Science), 29(1), pp.198-199.
PP-F40
Extraction,
Characterization and in-vivo Screening of Anti-inflammatory Activities of Lagas
camollis cav
Mukta Agrawal and Pratibha Sahu
Rungta College of Pharmaceutical
Sciences and Research, Kohka-Kurud Road, Bhilai - 490 024, India
Corresponding author email:
muktu0205@live.com
[Received
15 January 2016; accepted 29 January 2016]
Abstract: Currently
available synthetic anti-inflammatory drugs pose a major problem during their
clinical uses. Therefore, efforts are being made by several researchers to
develop novel herbal based anti-inflammatory drugs. Many plant species known in
folk medicine of different cultures used for their anti-inflammatory and
analgesic properties were proved to be good for the treatment of inflammatory
conditions and pain. The present study was aimed to identify the anti-Inflammatory
activity of leaves of Lagascamollis (Cav.) family Asteraceae. The active
ingredient was extracted by continuous hot extraction method using ethanol as
solvent. The extract was then lyophilized and the dried material was stored in
a desiccator. The extract was then subjected for different pharmacognostic,
phytochemical studies and different constituents like flavonoids, tannins were
estimated quantitatively. Male Wistar rats (250-260g) were chosen as a
pharmacological model to study the anti-inflammatory activity of the extract.In
first series of studies; acute oral toxicity study was performed. The extract
caused no mortality in rats and the extracts were found to be safe upto a dose
of 2000 mg/kg/bw. Based on toxicity study data, two doses of 200and 400mg/kg/bw
were selected for examining the biological activity. Carrageenan and Egg
albumin were selected as noxious agents to induce paw edema in rats.
Theethanolic, aqueous, ethyl acetate and n-butanol extracts shows significant
reduction in inflammation Further, as compared with standard drug diclofenac
(15 mg/kg), the effect was slightly better. Among all the extracts the
ethanolic extract at a dose of 400 mg/kg maximum suppression of paw edema after
120 minutes.In necropsy study, the injury at the site of administration was not
registered. Viewed collectively. the results of the present study showed that
ethanolic extract of the leaves of Lagascamollis exhibit potent
anti-inflammatory activity without producingany untoward effects.
Key words: Herbal medicine,
Lagascamollis, Anti-inflammatory, Carrageenan, Egg albumin